Product Name: SOD (EC)
Product Number: AB-NN328-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Extracellular superoxide dismutase [Cu-Zn]
Target Alias: EC SOD; EC-SOD; Extracellular superoxide dismutase; Extracellular superoxide dismutase precursor; MGC20077; SOD 3; SOD3; SODE_HUMAN; Superoxide dismutase 3 extracellular antibody
Product Type Specific: Superoxide dismutase pan-specific antibody
Antibody Code: NN328-1
Antibody Target Type: Pan-specific
Protein UniProt: P08294
Protein SigNET: P08294
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1 Kappa
Target Alias: EC SOD; EC-SOD; Extracellular superoxide dismutase; Extracellular superoxide dismutase precursor; MGC20077; SOD 3; SOD3; SODE_HUMAN; Superoxide dismutase 3 extracellular antibody
Product Type Specific: Superoxide dismutase pan-specific antibody
Antibody Code: NN328-1
Antibody Target Type: Pan-specific
Protein UniProt: P08294
Protein SigNET: P08294
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1 Kappa
Antibody Immunogen Source: Human extracellular SOD purified from aortas
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ELISA
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ELISA
Antibody Dilution Recommended: WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects an extracellular SOD ~35 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-167 was sufficient for detection of EC-SOD in 20 µg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-2)
Related Product 2: SOD (Cu/Zn) pan-specific antibody (Cat. No.: AB-NN098-3)
Related Product 3: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-1)
Antibody Potency: Detects an extracellular SOD ~35 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-167 was sufficient for detection of EC-SOD in 20 µg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-2)
Related Product 2: SOD (Cu/Zn) pan-specific antibody (Cat. No.: AB-NN098-3)
Related Product 3: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-1)
Related Product 4: SOD (Mn) pan-specific antibody (Cat. No.: AB-NN068-1)
Related Product 5: SOD (Mn) pan-specific antibody (Cat. No.: AB-NN068-2)
Related Product 6: SOD1 (UβB) pan-specific antibody (Cat. No.: AB-NN098-5)
Related Product 7: SOD1 (EDI) pan-specific antibody (Cat. No.: AB-NN098-4)
Related Product 5: SOD (Mn) pan-specific antibody (Cat. No.: AB-NN068-2)
Related Product 6: SOD1 (UβB) pan-specific antibody (Cat. No.: AB-NN098-5)
Related Product 7: SOD1 (EDI) pan-specific antibody (Cat. No.: AB-NN098-4)
Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4).
There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).
References
[1] Adachi T. et al. (1992) Clin Chim Acta. 212: 89-102.
[2] Barrister J.V. et al. (1987). Crit. Rev. Biochem. 22:111-180.
[3] FurukawaY., and O’Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6.
[4] Gao B. et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925.
[5] Hassan H.M. (1988) Free Radical Biol. Med. 5: 377-385.
[6] Wispe J.R. et al. (1989) BBA. 994: 30-36.
[7] Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491
[1] Adachi T. et al. (1992) Clin Chim Acta. 212: 89-102.
[2] Barrister J.V. et al. (1987). Crit. Rev. Biochem. 22:111-180.
[3] FurukawaY., and O’Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6.
[4] Gao B. et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925.
[5] Hassan H.M. (1988) Free Radical Biol. Med. 5: 377-385.
[6] Wispe J.R. et al. (1989) BBA. 994: 30-36.
[7] Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491
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