Product Name: KCC2
Product Number: AB-NN322-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Solute carrier family 12 member 5
Target Alias: Potassium Chloride Cotransporter; Potassium chloride transporter 5; SLC12A5; hKCC2; S12A5; Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; Furosemide-sensitive K-Cl cotransporter; K-Cl cotransporter 2; rKCC2; Neuronal K-Cl cotransporter; KIAA1176; Electroneutral potassium chloride cotransporter 2; Erythroid K Cl cotransporter 2; Furosemide sensitive K Cl cotransporter; K-Cl cotransporter 2; KCC 2; Neuronal K Cl cotransporter; Solute carrier family 12 (potassium chloride transporter) member 5
Product Type Specific: Potassium Chloride cotransport protein pan-specific antibody
Antibody Code: NN322-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9H2X9
Target Alias: Potassium Chloride Cotransporter; Potassium chloride transporter 5; SLC12A5; hKCC2; S12A5; Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; Furosemide-sensitive K-Cl cotransporter; K-Cl cotransporter 2; rKCC2; Neuronal K-Cl cotransporter; KIAA1176; Electroneutral potassium chloride cotransporter 2; Erythroid K Cl cotransporter 2; Furosemide sensitive K Cl cotransporter; K-Cl cotransporter 2; KCC 2; Neuronal K Cl cotransporter; Solute carrier family 12 (potassium chloride transporter) member 5
Product Type Specific: Potassium Chloride cotransport protein pan-specific antibody
Antibody Code: NN322-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9H2X9
Protein SigNET: Q9H2X9
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Fusion protein amino acids 932-1043 corresponding to rat KCC2
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Fusion protein amino acids 932-1043 corresponding to rat KCC2
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:300); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~140 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-392 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:300); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~140 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-392 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Scientific Background: KCC2 is a member of the cation-chloride cotransporter gene family (1). It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain (2, 3). KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord (4). Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
References
[1] Lee L.H., Walker J.A., Williams J.R., Goodier R.J., Payne J.A., Moss S.J. (2007) J Biol Chem. 282(41): 29777-29784.
[2] Watanabe M., Wake H., Moorhouse A.J., Nabekura J. (2009) J Biol Chem. 284(41): 27980-27988.
[3] Gulyas A.I., Sik A., Payne J.A., Kaila K., Freund T.F. (2001) Eur J Neurosci. 13(12): 2205-2217.
[4] Vinay L., Jean-Xavier C. (2008) Brain Res Rev. 57(1): 103-110.
[1] Lee L.H., Walker J.A., Williams J.R., Goodier R.J., Payne J.A., Moss S.J. (2007) J Biol Chem. 282(41): 29777-29784.
[2] Watanabe M., Wake H., Moorhouse A.J., Nabekura J. (2009) J Biol Chem. 284(41): 27980-27988.
[3] Gulyas A.I., Sik A., Payne J.A., Kaila K., Freund T.F. (2001) Eur J Neurosci. 13(12): 2205-2217.
[4] Vinay L., Jean-Xavier C. (2008) Brain Res Rev. 57(1): 103-110.
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