Product Name: NCC
Product Number: AB-NN3211
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Solute carrier family 12 member 3
Target Alias: SLC12A3; SCYL1;CKb10; MCP-4; MGC17134; NCC-1; NCC1; SCYA13; CK-beta-10; monocyte chemoattractant protein 4; monocyte chemotactic protein 4; new CC chemokine 1; small inducible cytokine A13; small inducible cytokine subfamily A (Cys-Cys) member 13; chemokine (C-C)
Product Type Specific: Chemokine pan-specific antibody
Antibody Code: NN3211
Antibody Target Type: Pan-specific
Protein UniProt: P55017
Protein SigNET: P55017
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Target Alias: SLC12A3; SCYL1;CKb10; MCP-4; MGC17134; NCC-1; NCC1; SCYA13; CK-beta-10; monocyte chemoattractant protein 4; monocyte chemotactic protein 4; new CC chemokine 1; small inducible cytokine A13; small inducible cytokine subfamily A (Cys-Cys) member 13; chemokine (C-C)
Product Type Specific: Chemokine pan-specific antibody
Antibody Code: NN3211
Antibody Target Type: Pan-specific
Protein UniProt: P55017
Protein SigNET: P55017
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Poduced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | IEM
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | IEM
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~160 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Dog
Antibody Positive Control: 1 µg/ml of SPC-402 was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Potency: Detects a ~160 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Dog
Antibody Positive Control: 1 µg/ml of SPC-402 was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Scientific Background: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
References
[1] Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712.
[2] Tiwari S. et al. (2009) Am J Nephrol. 30(6): 554-562.
[3] Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.
[1] Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712.
[2] Tiwari S. et al. (2009) Am J Nephrol. 30(6): 554-562.
[3] Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.
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