Product Name: SCNN1B (NaC beta)
Product Number: AB-NN317-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Amiloride sensitive sodium channel subunit beta
Target Alias: SCNN1B; Amiloride-sensitive sodium channel beta-subunit; Beta ENaC; Beta NaCH; ENaC beta; ENaCB; Epithelial Na(+) channel subunit beta; Epithelial Na+ channel beta subunit; Epithelial Na+ channel subunit beta; Epithelial sodium channel beta 2 subunit; Epithelial sodium channel beta 3 subunit; Nonvoltage gated sodium channel 1 beta subunit; Nonvoltage gated sodium channel 1 subunit beta; Nonvoltage-gated sodium channel 1 beta subunit; SCNEB; SCNN 1B; Sodium channel nonvoltage gated 1 beta (Liddle syndrome); Sodium channel nonvoltage gated 1 beta
Product Type Specific: Sodium channel pan-specific antibody
Antibody Code: NN317-2
Antibody Target Type: Pan-specific
Protein UniProt: P51168
Target Alias: SCNN1B; Amiloride-sensitive sodium channel beta-subunit; Beta ENaC; Beta NaCH; ENaC beta; ENaCB; Epithelial Na(+) channel subunit beta; Epithelial Na+ channel beta subunit; Epithelial Na+ channel subunit beta; Epithelial sodium channel beta 2 subunit; Epithelial sodium channel beta 3 subunit; Nonvoltage gated sodium channel 1 beta subunit; Nonvoltage gated sodium channel 1 subunit beta; Nonvoltage-gated sodium channel 1 beta subunit; SCNEB; SCNN 1B; Sodium channel nonvoltage gated 1 beta (Liddle syndrome); Sodium channel nonvoltage gated 1 beta
Product Type Specific: Sodium channel pan-specific antibody
Antibody Code: NN317-2
Antibody Target Type: Pan-specific
Protein UniProt: P51168
Protein SigNET: P51168
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Synthetic peptide from the C-terminal of Rat ENaC beta (AA 617-638)
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Synthetic peptide from the C-terminal of Rat ENaC beta (AA 617-638)
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a weak to medium ~87 kDa protein band in rat kidney lysates.
Antibody Species Reactivity: Mouse
Antibody Positive Control: A 1:1000 dilution of SMC-241 was sufficient for detection of ENaC beta in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-high
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a weak to medium ~87 kDa protein band in rat kidney lysates.
Antibody Species Reactivity: Mouse
Antibody Positive Control: A 1:1000 dilution of SMC-241 was sufficient for detection of ENaC beta in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-high
Antibody Cross Reactivity: Appears to cross-react with lower molecular mass (<60 kDa) in mouse brain lysates.
Scientific Background: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2).
ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
References
[1] Kakizoe Y. et al. (2009) J Hpyertens. 27(8): 1679-1689.
[2] Gu Y. (2008) J Cell Physiol. 216(2):453-457.
[3] Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609.
[4] Mauro T. et al. (2002) J Invest Dermatol. 118(4): 589-594.
[5] Elias N. et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45.
[6] Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
[1] Kakizoe Y. et al. (2009) J Hpyertens. 27(8): 1679-1689.
[2] Gu Y. (2008) J Cell Physiol. 216(2):453-457.
[3] Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609.
[4] Mauro T. et al. (2002) J Invest Dermatol. 118(4): 589-594.
[5] Elias N. et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45.
[6] Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
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