Product Name: ENaC Alpha
Product Number: AB-NN316-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Amiloride-sensitive sodium channel alpha1 subunit
Target Alias: SCNN1A; Epithelial Sodium Channel-α; Epithelial Sodium Channel alpha; Alpha ENaC 2; Alpha ENaC; Alpha NaCH; Alpha-ENaC; Amiloride sensitive epithelial sodium channel alpha subunit; Amiloride sensitive sodium channel subunit alpha; ENaCa; ENaCalpha; Epithelial Na(+) channel subunit alpha; Epithelial Na+ channel subunit alpha; FLJ21883; Nonvoltage gated sodium channel 1 subunit alpha; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; SCNN 1; SCNN1; SCNN1A; SCNNA_HUMAN; Sodium channel nonvoltage gated 1 alpha
Product Type Specific: Sodium channel pan-specific antibody
Antibody Code: NN316-1
Antibody Target Type: Pan-specific
Protein UniProt: P37088
Target Alias: SCNN1A; Epithelial Sodium Channel-α; Epithelial Sodium Channel alpha; Alpha ENaC 2; Alpha ENaC; Alpha NaCH; Alpha-ENaC; Amiloride sensitive epithelial sodium channel alpha subunit; Amiloride sensitive sodium channel subunit alpha; ENaCa; ENaCalpha; Epithelial Na(+) channel subunit alpha; Epithelial Na+ channel subunit alpha; FLJ21883; Nonvoltage gated sodium channel 1 subunit alpha; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; SCNN 1; SCNN1; SCNN1A; SCNNA_HUMAN; Sodium channel nonvoltage gated 1 alpha
Product Type Specific: Sodium channel pan-specific antibody
Antibody Code: NN316-1
Antibody Target Type: Pan-specific
Protein UniProt: P37088
Protein SigNET: P37088
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Produced against a synthetic peptide mapping to the N-temrinal of the alpha subunit (amino acids 46-68) of rat Alpha ENaC (antibody designation 3560-2).
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Produced against a synthetic peptide mapping to the N-temrinal of the alpha subunit (amino acids 46-68) of rat Alpha ENaC (antibody designation 3560-2).
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~85 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Rat | Mouse
Antibody Positive Control: 1 µg/ml of SPC-403 was sufficient for detection of alpha-ENaC in 35 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~85 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Rat | Mouse
Antibody Positive Control: 1 µg/ml of SPC-403 was sufficient for detection of alpha-ENaC in 35 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Medium
Related Product 1: ENaC Beta pan-specific antibody (Cat. No.: AB-NN317-1)
Related Product 2: ENaC Gamma pan-specific antibody (Cat. No.: AB-NN369-1)
Related Product 1: ENaC Beta pan-specific antibody (Cat. No.: AB-NN317-1)
Related Product 2: ENaC Gamma pan-specific antibody (Cat. No.: AB-NN369-1)
Scientific Background: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2).
ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
References
[1] Kakizoe Y. et al. (2009) J Hpyertens. 27(8): 1679-1689.
[2] Gu Y. (2008) J Cell Physiol. 216(2):453-457.
[3] Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609.
[4] Mauro T. et al. (2002) J Invest Dermatol. 118(4): 589-594.
[5] Elias N. et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45.
[6] Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
[1] Kakizoe Y. et al. (2009) J Hpyertens. 27(8): 1679-1689.
[2] Gu Y. (2008) J Cell Physiol. 216(2):453-457.
[3] Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609.
[4] Mauro T. et al. (2002) J Invest Dermatol. 118(4): 589-594.
[5] Elias N. et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45.
[6] Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
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