Product Name: Rhodopsin
Product Number: AB-NN313-2
Size: 25 µg      Price:89.00
      $US
Target Full Name: Rhodopsin

Target Alias: OPN2; opsd; opsin 2; opsin 2 rod pigment; opsin2; RHO; RP4; MGC138309; Retinitis Pigmentosa 4

Product Type Specific: G protein-coupled receptor pan-specific antibody

Antibody Code: NN313-2

Antibody Target Type: Pan-specific

Protein UniProt: P08100

Protein SigNET: P08100

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Bovine Rhodopsin

Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA

Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody Potency: In mouse brain lysates, this antibody detects a ~40 kDa protein by Western blotting. Binds specifically to the N-terminus of Rhodopsin.

Antibody Species Reactivity: Vertebrates

Antibody Positive Control: 1 µg/ml of SMC-177 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Antibody Specificity: Very high

Antibody Cross Reactivity: Does not detect Rhodopsin in invertebrates.

Related Product 1: Rhodopsin pan-specific antibody (Cat. No.: AB-NN313-1)

Scientific Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
References

[1] Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61.

[2] Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867.

[3] Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875.

[4] Feurstein S.E. et al. (2009) Biochemistry. 48(45): 10733-10742.

[5] Iannaccone A. et al. (2006) Vision Res. 46(27): 4556-4567.