Product Name: PUMA (CT)
Product Number: AB-NN310-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Carbohydrate sulfotransferase 9
Target Alias: BBC3; BCL2 binding component 3; p53 up regulated modulator of apoptosis; PUMA/JFY1
Product Type Specific: Apoptosis protein pan-specific antibody
Antibody Code: NN310-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9BXH4
Protein SigNET: Q9BXH4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: C-terminal amino acids of human PUMA
Target Alias: BBC3; BCL2 binding component 3; p53 up regulated modulator of apoptosis; PUMA/JFY1
Product Type Specific: Apoptosis protein pan-specific antibody
Antibody Code: NN310-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9BXH4
Protein SigNET: Q9BXH4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: C-terminal amino acids of human PUMA
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 0.02% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 0.02% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~23 kDa protein in cell and tissue lysates by Western blotting. Detects a ~16 kDa protein bands sometimes, possibly corresponding to PUMAβ.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: 1 µg/ml of SPC-166 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Medium
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: 1 µg/ml of SPC-166 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Medium
Scientific Background: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
References
[1] Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94.
[2] Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82.
[3] Han J. et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.
[1] Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94.
[2] Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82.
[3] Han J. et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.
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