Product Name: p23
Product Number: AB-NN309-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Prostaglandin E synthase 3
Target Alias: co chaperone p23; PTGES3; TEBP; telomerase binding protein p23; unactive progesterone receptor 23kDa; HSP90 co-chaperone; Progesterone receptor complex p23; Cytosolic prostaglandin E2 synthase
Product Type Specific: p23 pan-specific antibody
Antibody Code: NN309-1
Antibody Target Type: Pan-specific
Protein UniProt: Q15185
Protein SigNET: Q15185
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Target Alias: co chaperone p23; PTGES3; TEBP; telomerase binding protein p23; unactive progesterone receptor 23kDa; HSP90 co-chaperone; Progesterone receptor complex p23; Cytosolic prostaglandin E2 synthase
Product Type Specific: p23 pan-specific antibody
Antibody Code: NN309-1
Antibody Target Type: Pan-specific
Protein UniProt: Q15185
Protein SigNET: Q15185
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Recombinant human full length p23 protein
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:2000) IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~23 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rabbit | Chicken | Guinea pig | S. cerevisiae
Antibody Positive Control: 0.5 µg/ml of SMC-156 was sufficient for detection of p23 in 20 µg of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Potency: High potency. Detects a ~23 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rabbit | Chicken | Guinea pig | S. cerevisiae
Antibody Positive Control: 0.5 µg/ml of SMC-156 was sufficient for detection of p23 in 20 µg of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the HSP90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the HSP90-progesterone receptor complex along with HSP70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate–mediated HSP90 binding of client proteins (8). Since many HSP90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). HSP90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology.
References
[1] Johnson J.L., Beito T. G., Krco C.J. & Toft D.O. (1994) Mol Cell Biol 14: 1956-63.
[2] Weikl T., Abelmann K. & Buchner J. (1999) J Mol Biol 293: 685-91.
[3] Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A. & Toft, D.O. (2000) J Biol Chem 275: 23045-52.
[4] Nair S.C. et al. (1996) Cell Stress Chaperones 1: 237-50.
[5] Xu Z. et al. (1997) Eur J Biochem 246, 461-70.
[6] Holt S.E. et al. (1999) Genes Dev 13: 817-26.
[7] Hu J., Toft D., Anselmo D. & Wang X. (2002) J Virol 76: 269-79.
[8] Felts, S.J. & Toft D.O. (2003) Cell Stress Chaperones 8: 108-13.
[1] Johnson J.L., Beito T. G., Krco C.J. & Toft D.O. (1994) Mol Cell Biol 14: 1956-63.
[2] Weikl T., Abelmann K. & Buchner J. (1999) J Mol Biol 293: 685-91.
[3] Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A. & Toft, D.O. (2000) J Biol Chem 275: 23045-52.
[4] Nair S.C. et al. (1996) Cell Stress Chaperones 1: 237-50.
[5] Xu Z. et al. (1997) Eur J Biochem 246, 461-70.
[6] Holt S.E. et al. (1999) Genes Dev 13: 817-26.
[7] Hu J., Toft D., Anselmo D. & Wang X. (2002) J Virol 76: 269-79.
[8] Felts, S.J. & Toft D.O. (2003) Cell Stress Chaperones 8: 108-13.
© Kinexus Bioinformatics Corporation 2017