Product Name: NPAS4
Product Number: AB-NN299-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Neuronal PAS domain-containing protein 4
Target Alias: Neuronal PAS4; Class E basic helix-loop-helix protein 79; HLH-PAS transcription factor NXF; PAS domain-containing protein 10; Limbic-enhanced PAS protein; bHLHe79; PASD10 and LE-PAS
Product Type Specific: Transcription factor pan-specific antibody
Antibody Code: NN299-1
Antibody Target Type: Pan-specific
Protein UniProt: Q8IUM7
Protein SigNET: Q8IUM7
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Target Alias: Neuronal PAS4; Class E basic helix-loop-helix protein 79; HLH-PAS transcription factor NXF; PAS domain-containing protein 10; Limbic-enhanced PAS protein; bHLHe79; PASD10 and LE-PAS
Product Type Specific: Transcription factor pan-specific antibody
Antibody Code: NN299-1
Antibody Target Type: Pan-specific
Protein UniProt: Q8IUM7
Protein SigNET: Q8IUM7
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 597-802 (C-terminus) of rat Npas4.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~90 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: A 1:100 dilution of SMC-495 was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Potency: Detects a ~90 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: A 1:100 dilution of SMC-495 was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Scientific Background: NPAS4 belongs to the Per-Arnt-Sim family of neuronal specific transciption factors, all involved in the development and maintenance of learning and memory pathways (1). NPAS4 is a transcription factor that regulates the formation and maintenance of inhibitory synapses, and can activate the CNS midline enhancer element and the expression of the drebrin gene. Deregulation has been to be associated with developmental disorders such as schizophrenia and autism (2).
References
[1] Zhaohui Z. et al. (2014) Exp Ther Med. 7(4): 1045-1049.
[2] Coutellier L. et al. (2012) Plos One. 7(9): e46604.
[1] Zhaohui Z. et al. (2014) Exp Ther Med. 7(4): 1045-1049.
[2] Coutellier L. et al. (2012) Plos One. 7(9): e46604.
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