Product Name: NALCN
Product Number: AB-NN293-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Sodium leak channel non-selective protein
Target Alias: VGCNL1; CanIon; Voltage gated channel-like protein 1; A530023G15Rik; bA430M15.1; FLJ23913; FLJ44659; FLJ44764; Four repeat voltage gated ion channel; MGC74524; Putative 4 repeat voltage gated ion channel; Sodium leak channel non-selective protein
Product Type Specific: Sodium channel pan-specific antibody
Antibody Code: NN293-1
Antibody Target Type: Pan-specific
Protein UniProt: Q8IZF0
Protein SigNET: Q8IZF0
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Target Alias: VGCNL1; CanIon; Voltage gated channel-like protein 1; A530023G15Rik; bA430M15.1; FLJ23913; FLJ44659; FLJ44764; Four repeat voltage gated ion channel; MGC74524; Putative 4 repeat voltage gated ion channel; Sodium leak channel non-selective protein
Product Type Specific: Sodium channel pan-specific antibody
Antibody Code: NN293-1
Antibody Target Type: Pan-specific
Protein UniProt: Q8IZF0
Protein SigNET: Q8IZF0
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~200 kDa protein by Western blotting.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-417 was sufficient for detection of NALCN in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP asthe secondary antibody. immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Potency: In mouse brain lysates, this antibody detects a ~200 kDa protein by Western blotting.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-417 was sufficient for detection of NALCN in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP asthe secondary antibody. immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: NALCN (sodium leak channel non-selective protein), alsoknown as CanIon or VGCNL1 (voltage gated channel-like protein 1), is a 1738 amino acid multi-pass membrane protein that belongs to the cation-nonselective channel family. NALCN is highly conserved in mammals and is widely expressed in the central nervous system. Activated by NK-1R, NALCN is a voltage-independent, nonselective cation channel which is permeable to sodium, potassium and calcium ions. NALCN is responsible for background sodium ion leak conductance in neurons and regulates basal excitability of the nervous systems. Defects of NALCN in mice causes disruption in respiratory rhythm and death occurs within 24 hours of birth. Three isoforms of NALCN exists due to alternative splicing events.
References
[1] Lee, J.H., Cribbs, L.L. and Perez-Reyes, E. (1999)FEBS Lett. 445: 231-236.
[2] Lu, B., Su, Y., Das, S., Liu, J., Xia, J. and Ren, D. (2007) Cell 129: 371-383.
[3] Jospin, M. et al. (2007) Curr. Biol. 17: 1595-1600.
[4] Gaultier, C. and Gallego, J. (2008) Appl. Physiol. 104: 1522-1530.
[5] Yeh, E. et al. (2008) PLoS Biol. 6: e55.
[1] Lee, J.H., Cribbs, L.L. and Perez-Reyes, E. (1999)FEBS Lett. 445: 231-236.
[2] Lu, B., Su, Y., Das, S., Liu, J., Xia, J. and Ren, D. (2007) Cell 129: 371-383.
[3] Jospin, M. et al. (2007) Curr. Biol. 17: 1595-1600.
[4] Gaultier, C. and Gallego, J. (2008) Appl. Physiol. 104: 1522-1530.
[5] Yeh, E. et al. (2008) PLoS Biol. 6: e55.
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