Target Full Name: Metabotropic glutamate receptor 5

Target Alias: Glutamate receptor metabotropic 5;GPRC1E; GRM 5; GRM5; Metabotropic glutamate receptor 5 variant F; Metabotropic glutamate receptor 5 variant G; Metabotropic glutamate receptor 5 variant H; mGlu5; mGluR5; mGluR5a; mGluR5b; Glutamate receptor metabotropic 1; glutamate receptor; metabotropic 1; GPRC1A; GRM 1; GRM1 alpha; GRM1; GRM1_HUMAN; GRM1A; Metabotropic glutamate receptor 1; mGlu1; mGluR1 alpha; mGluR1; mGluR1A

Product Type Specific: G protein-coupled receptor pan-specific antibody

Antibody Code: NN291-2

Antibody Target Type: Pan-specific

Protein UniProt: P41594

Protein SigNET: P41594

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation

Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a ~130 kDa protein (both mGluR1 and mGluR5).

Antibody Species Reactivity: Human | Mouse | Rat

Antibody Positive Control: 1 µg/ml of SMC-407 was sufficient for detection of mGluR1/5 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Related Product 1: mGluR1/5 glutamate receptor pan-specific antibody (Cat. No.: AB-NN291-1)