Target Full Name: Metabotropic glutamate receptor 5

Target Alias: mGluR; metabotropic glutamate receptor 1; mGlu5; mGlu1; mGluR5; mGluR1; GPRC1E; GRM5; Metabotropic glutamate receptor 5 variant F; Metabotropic glutamate receptor 5 variant G; Metabotropic glutamate receptor 5 variant H; Glutamate receptor metabotropic 5

Product Type Specific: G protein-coupled receptor pan-specific antibody

Antibody Code: NN291-1

Antibody Target Type: Pan-specific

Protein UniProt: P41594

Protein SigNET: P41594

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG2A

Antibody Immunogen Source: Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation

Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.

Antibody Potency: In mouse brain lysates, this antibody detects a ~130 kDa protein (both mGluR1 and mGluR5).

Antibody Species Reactivity: Human | Mouse | Rat

Antibody Positive Control: 1 µg/ml of SMC-419 was sufficient for detection of mGluR1/5 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Antibody Specificity: High

Antibody Cross Reactivity: One strong cross-reactive protein at ~65 kDa in mouse brain lysates.