Product Name: LAMP1
Product Number: AB-NN284-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Lysosome-associated membrane glycoprotein 1
Target Alias: CD107; CD107a; LAMPA; LGP120; IgpA; CD107 antigen-like family member A; 120 kDa lysosomal membrane glycoprotein; Lamp-1
Product Type Specific: LAMP1 pan-specific antibody
Antibody Code: NN284-1
Antibody Target Type: Pan-specific
Protein UniProt: P11279
Protein SigNET: P11279
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Rat liver lysosomal membrane preparations
Target Alias: CD107; CD107a; LAMPA; LGP120; IgpA; CD107 antigen-like family member A; 120 kDa lysosomal membrane glycoprotein; Lamp-1
Product Type Specific: LAMP1 pan-specific antibody
Antibody Code: NN284-1
Antibody Target Type: Pan-specific
Protein UniProt: P11279
Protein SigNET: P11279
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Rat liver lysosomal membrane preparations
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~120 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Rat | Hamster
Antibody Positive Control: 1 µg/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.
Related Product 1: LAMP2 pan-specific antibody (Cat. No.: AB-NN284-2)
Antibody Species Reactivity: Rat | Hamster
Antibody Positive Control: 1 µg/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.
Related Product 1: LAMP2 pan-specific antibody (Cat. No.: AB-NN284-2)
Scientific Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3).
This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).
References
[1] Grutzkau, A. et al (2004) Cytometry A. 61(10): 62-68.
[2] Furuta, K. et al. (1999) EMBO J. 17(5):1304-14.
[3] Rohrer, J. et al. (1996) J Cell Biol. 132(4): 565-76.
[4] Kannan, K. et al. (1996) Cell Immunol. 171: 10-19.
[5] Lewis, V. et al. (1985) J. Cell Biol. 100: 1839-1847.
[6] Jones, K.A. et al. (2004) Exp Cell Res 295(2): 512-524.
[1] Grutzkau, A. et al (2004) Cytometry A. 61(10): 62-68.
[2] Furuta, K. et al. (1999) EMBO J. 17(5):1304-14.
[3] Rohrer, J. et al. (1996) J Cell Biol. 132(4): 565-76.
[4] Kannan, K. et al. (1996) Cell Immunol. 171: 10-19.
[5] Lewis, V. et al. (1985) J. Cell Biol. 100: 1839-1847.
[6] Jones, K.A. et al. (2004) Exp Cell Res 295(2): 512-524.
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