Product Name: Ras
Product Number: AB-NN281-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: GTPases Hras and KRas

Target Alias: GTPase HRas; GTPase KRas; GTPase NRas; Ha Ras; K Ras; K ras p21; KRAS; NRAS; p21ras; RASH2

Product Type Specific: G (Ras) protein pan-specific antibody

Antibody Code: NN281-1

Antibody Target Type: Pan-specific

Protein UniProt: P01116

Protein SigNET: P01116

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Ig Isotype Clone: N/A

Antibody Immunogen Source: Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fingi, Xenopus, rat, mouse and chicken over these residues.)

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence

Antibody Dilution Recommended: WB (1:1000), IHC (1:100), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a ~21 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human | Mouse | Rat | Bovine

Antibody Positive Control: 1 µg/ml of SPC-173 was sufficient for detection of Ras in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Scientific Background: The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
References

[1] Boguski M.S. and McCormick F. (1993) Nature 366: 643–654.

[2] Avruch J. et al. (1994) Trends Biochem. Sci.19: 279–283.

[3] Buday L. and Downward J. (1993) Cell 73: 611–620.

[4] Huang D.C. et al. (1993) Mol. Cell Biol. 13: 2420–2431.

[5] Bos J.L. (1989) Cancer Res. 49: 4682–4689.

[6] Ma J. and Karplus M. (1997) J. Mol. Biol. 274:114–131.