Product Name: Kir6.1
Product Number: AB-NN280-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: ATP-sensitive inward rectifier potassium channel 8
Target Alias: Potassium channel inwardly rectifying subfamily J member 8; uKATP-1; Kcnj8
Product Type Specific: Potassium channel pan-specific antibody
Antibody Code: NN280-1
Antibody Target Type: Pan-specific
Protein UniProt: Q15842
Protein SigNET: Q15842
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2A
Antibody Immunogen Source: Fusion protein amino acids 306-424 (Cytoplasmic C-terminus) of rat Kir6.1
Target Alias: Potassium channel inwardly rectifying subfamily J member 8; uKATP-1; Kcnj8
Product Type Specific: Potassium channel pan-specific antibody
Antibody Code: NN280-1
Antibody Target Type: Pan-specific
Protein UniProt: Q15842
Protein SigNET: Q15842
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2A
Antibody Immunogen Source: Fusion protein amino acids 306-424 (Cytoplasmic C-terminus) of rat Kir6.1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~45 kDa protein by Western blotting.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: A 1:100 dilution of SMC-491 was sufficient for detection of Kir6.1 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: A 1:100 dilution of SMC-491 was sufficient for detection of Kir6.1 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family (1-3). This is predominantly detected in fetal and adult hearts, and defects can be associated with J-wave syndromes, a group of heart disorders characterized by early repolarization events (4).
References
[1] Zobel C. et al. (2003) J Physiol. 550: 365-372.
[2] Panama B.K., McLerie M., and Lopatin A.N. (2007) Am J Physiol Heart Circ Physiol. 293: H3558-H3567.
[3] Munoz V. et al. (2007) Heart Rhythm. 4(4): 487-496.
[4] Aguilar-Bryan L. et al. (1998) Physiol Rev. 78(1): 227-245.
[1] Zobel C. et al. (2003) J Physiol. 550: 365-372.
[2] Panama B.K., McLerie M., and Lopatin A.N. (2007) Am J Physiol Heart Circ Physiol. 293: H3558-H3567.
[3] Munoz V. et al. (2007) Heart Rhythm. 4(4): 487-496.
[4] Aguilar-Bryan L. et al. (1998) Physiol Rev. 78(1): 227-245.
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