Product Name: GIT1
Product Number: AB-NN261-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: ARF GTPase-activating protein GIT1
Target Alias: ARF GAP GIT1; ARF GTPase-activating protein; CAT1; GRK-interacting protein 1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase-interactor 1; GIT 1
Product Type Specific: GTPase-activating protein pan-specific antibody
Antibody Code: NN261-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y2X7
Protein SigNET: Q9Y2X7
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Target Alias: ARF GAP GIT1; ARF GTPase-activating protein; CAT1; GRK-interacting protein 1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase-interactor 1; GIT 1
Product Type Specific: GTPase-activating protein pan-specific antibody
Antibody Code: NN261-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y2X7
Protein SigNET: Q9Y2X7
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunoprecipitation
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~90 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-413 was sufficient for detection of GIT1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
Antibody Cross Reactivity: Does not cross-react with GIT2.
Related Product 1: GIT1-pY545 phosphosite-specific antibody (Cat. No.: AB-PN517)
Antibody Potency: Detects a ~90 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-413 was sufficient for detection of GIT1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
Antibody Cross Reactivity: Does not cross-react with GIT2.
Related Product 1: GIT1-pY545 phosphosite-specific antibody (Cat. No.: AB-PN517)
Scientific Background: G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration (2). GIT-1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington disease (4). Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains (5). Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology (4).
References
[1] Hoefen R.J. and Berk B.C. (2006) J. Cell Sci. 119: 146 1475.
[2] Manabe R. et al. (2002) J. Cell Sci. 115: 1497-1510.
[3] Zhang H. et al. (2003) J. Cell Biol. 161: 131-142.
[4] Goehler H. et al. (2004) Mol. Cell 15: 853-865.
[5] Mende-Mueller L.M. et al. (2001) J. Neurosci. 21: 1830-1837.
[1] Hoefen R.J. and Berk B.C. (2006) J. Cell Sci. 119: 146 1475.
[2] Manabe R. et al. (2002) J. Cell Sci. 115: 1497-1510.
[3] Zhang H. et al. (2003) J. Cell Biol. 161: 131-142.
[4] Goehler H. et al. (2004) Mol. Cell 15: 853-865.
[5] Mende-Mueller L.M. et al. (2001) J. Neurosci. 21: 1830-1837.
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