Product Name: FKBP51
Product Number: AB-NN249-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Peptidyl-prolyl cis-trans isomerase FKBP5
Target Alias: AIG6; FK506 binding protein 5; FKBP5; FKBP54; HSP90 binding immunophilin; p54; Pplase; Ptg10; Rotamase; T cekk FK506 binding protein
Product Type Specific: FK506 binding protein pan-specific antibody
Antibody Code: NN249-1
Antibody Target Type: Pan-specific
Protein UniProt: Q13451
Protein SigNET: Q13451
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG
Target Alias: AIG6; FK506 binding protein 5; FKBP5; FKBP54; HSP90 binding immunophilin; p54; Pplase; Ptg10; Rotamase; T cekk FK506 binding protein
Product Type Specific: FK506 binding protein pan-specific antibody
Antibody Code: NN249-1
Antibody Target Type: Pan-specific
Protein UniProt: Q13451
Protein SigNET: Q13451
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Synthetic peptide corresponding to the residues of human FKBP51
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~60 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Rabbit | Dog | Hamster
Antibody Positive Control: 1 µg/ml of SMC-461 was sufficient for detection of LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: FKBP52 pan-specific antibody (Cat. No.: AB-NN127)
Antibody Potency: Detects a ~60 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Rabbit | Dog | Hamster
Antibody Positive Control: 1 µg/ml of SMC-461 was sufficient for detection of LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: FKBP52 pan-specific antibody (Cat. No.: AB-NN127)
Scientific Background: HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3).
The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3).
FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
References
[1] Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F.(2003) J. Biol. Chem. 278(19): 17388-17394.
[2] Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600.
[3] Wu B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353.
[4] Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7): 3194-3201.
[1] Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F.(2003) J. Biol. Chem. 278(19): 17388-17394.
[2] Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600.
[3] Wu B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353.
[4] Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7): 3194-3201.
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