Product Name: FIH
Product Number: AB-NN248-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Hypoxia-inducible factor 1-alpha inhibitor
Target Alias: Factor inhibiting HIF1 (hypoxia-inducible factor); DKFZp762F1811; Factor inhibiting HIF-1; Factor inhibiting HIF1; FIH 1; FIH-1; FIH1; FLJ20615; FLJ22027; HIF1AN; HIF1N_HUMAN; Hypoxia inducible factor 1 alpha subunit inhibitor; Hypoxia inducible factor asparagine hydroxylase; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; Peptide aspartate beta dioxygenase
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN248-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9NWT6
Protein SigNET: Q9NWT6
Antibody Type: Monoclonal
Target Alias: Factor inhibiting HIF1 (hypoxia-inducible factor); DKFZp762F1811; Factor inhibiting HIF-1; Factor inhibiting HIF1; FIH 1; FIH-1; FIH1; FLJ20615; FLJ22027; HIF1AN; HIF1N_HUMAN; Hypoxia inducible factor 1 alpha subunit inhibitor; Hypoxia inducible factor asparagine hydroxylase; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; Peptide aspartate beta dioxygenase
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN248-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9NWT6
Protein SigNET: Q9NWT6
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Full length human FIH expressed in E.coli BL21 (DE3) cells
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Full length human FIH expressed in E.coli BL21 (DE3) cells
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:100), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium potency. Detects a ~45 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: 1 µg/ml of SMC-182 was sufficient for detection of FIH in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-low
Antibody Dilution Recommended: WB (1:100), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium potency. Detects a ~45 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: 1 µg/ml of SMC-182 was sufficient for detection of FIH in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-low
Scientific Background: FIH, Factor inhibiting HIF1 (hypoxia-inducible factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL represses HIF-1 transcriptional activity by disrupting the interaction of HIF-1 with the transcriptional co-activators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia (1-3). Recent studies show that low nuclear expression of FIH is a strong independent prognostic factor for a poor overall survival in clear cell renal cell carcinoma (4).
References
[1] Stolze I.P. et al. (2004) J Bio Chem. 42719-4272
[2] Soilleux E.J. et al. (2005) Histopathology 47:602-610.
[3] Moon H., Han S., Park H., Choe J. (2010) Mol Cells. 29(5): 471-474.
[5] Kroeze S.G. et al. (2010) Eur J Cancer. Epub.
[1] Stolze I.P. et al. (2004) J Bio Chem. 42719-4272
[2] Soilleux E.J. et al. (2005) Histopathology 47:602-610.
[3] Moon H., Han S., Park H., Choe J. (2010) Mol Cells. 29(5): 471-474.
[5] Kroeze S.G. et al. (2010) Eur J Cancer. Epub.
© Kinexus Bioinformatics Corporation 2017