Product Name: DUX4
Product Number: AB-NN243-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Double homeobox protein 4
Target Alias: Double homeobox 4; Double homeobox 10; DUX10; Double homeobox protein 4/10
Product Type Specific: DUX4 pan-specific antibody
Antibody Code: NN243-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UBX2
Protein SigNET: Q9UBX2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag
Target Alias: Double homeobox 4; Double homeobox 10; DUX10; Double homeobox protein 4/10
Product Type Specific: DUX4 pan-specific antibody
Antibody Code: NN243-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UBX2
Protein SigNET: Q9UBX2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~45 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: 1ug/ml of SMC-192 was sufficient for detection of DUX4 in 20ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary.
Antibody Cross Reactivity: No cross-reactivity with DUX4c.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: 1ug/ml of SMC-192 was sufficient for detection of DUX4 in 20ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary.
Antibody Cross Reactivity: No cross-reactivity with DUX4c.
Scientific Background: DUX4, or double homeobox4, is a human protein that is a transcriptional activator of paired-like homeodomain transcription factor 1 (1). Clinically it is a facioscapulohumeral muscular dystrophy candidate gene that appears to have a toxic gain of function (2-4). In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle and possibly other differentiated tissues (5).
References
[1] Entrez Gene: “Dux4 Double Homeobox, 4”
[2] Dixit M. et al., (2007) Proc. Natl Acad Sci. USA. 104(46): 18157-18162.
[3] Kowaljow V. et al. (2007) Neuromuscl Disord. 17(8): 611-623.
[4] Lemmers R. et al. (2010) Science Express. 329(5999): 1650-1653.
[5] Snider L. et al. (2010) PLoS Genetics. 6:1-14.
[1] Entrez Gene: “Dux4 Double Homeobox, 4”
[2] Dixit M. et al., (2007) Proc. Natl Acad Sci. USA. 104(46): 18157-18162.
[3] Kowaljow V. et al. (2007) Neuromuscl Disord. 17(8): 611-623.
[4] Lemmers R. et al. (2010) Science Express. 329(5999): 1650-1653.
[5] Snider L. et al. (2010) PLoS Genetics. 6:1-14.
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