Product Name: DNMT3L
Product Number: AB-NN242-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: DNA (cytosine-5)-methyltransferase 3-like
Target Alias: Cytosine 5 methyltransferase 3 like protein; DNA (cytosine-5)-methyltransferase 3-like; DNA cytosine 5 methyltransferase 3 like protein; DNA methyltransferase 3 like protein; DNM3L_HUMAN; Dnmt 3L; Dnmt3l; Human cytosine 5 methyltransferase 3 like protein; MGC1090 antibody
Product Type Specific: DNA methyltransferase pan-specific antibody
Antibody Code: NN242-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UJW3
Protein SigNET: Q9UJW3
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Target Alias: Cytosine 5 methyltransferase 3 like protein; DNA (cytosine-5)-methyltransferase 3-like; DNA cytosine 5 methyltransferase 3 like protein; DNA methyltransferase 3 like protein; DNM3L_HUMAN; Dnmt 3L; Dnmt3l; Human cytosine 5 methyltransferase 3 like protein; MGC1090 antibody
Product Type Specific: DNA methyltransferase pan-specific antibody
Antibody Code: NN242-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UJW3
Protein SigNET: Q9UJW3
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 1-387 of human DNMT3L
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Immunogen Source: Fusion protein amino acids 1-387 of human DNMT3L
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~45 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: 1 µg/ml of SMC-341 was sufficient for detection of DNMT3L in 10 µg of COS cell transiently transfected with Flag-tagged DNMT3L lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Medium
Antibody Cross Reactivity: No cross-reactivity against DNMT3a or DNMT3B.
Antibody Potency: High potency. Detects a ~45 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: 1 µg/ml of SMC-341 was sufficient for detection of DNMT3L in 10 µg of COS cell transiently transfected with Flag-tagged DNMT3L lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Medium
Antibody Cross Reactivity: No cross-reactivity against DNMT3a or DNMT3B.
Scientific Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8).
References
[1] Bestor T. et al.(1988) J.Mol. Biol. 203: 971-983.
[2] Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl. Acids Res. 20: 2287-2291.
[3] Xie S. et al. (1999) Gene 236: 87-95.
[4] Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257.
[5] Reik W. et al. (1999) J. Nat. Genet 23: 380-382.
[6] Robertson K.D. et al. (2000) Nat Genet 25(3): 338-342.
[7] Kareta MS. et al. (2006) J Biol Chem. 281(36): 25893-25902.
[8] Gokul G., Ramakrishna G., and Khosla S. (2009) Epigenetics. 4(5): 322-329.
[1] Bestor T. et al.(1988) J.Mol. Biol. 203: 971-983.
[2] Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl. Acids Res. 20: 2287-2291.
[3] Xie S. et al. (1999) Gene 236: 87-95.
[4] Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257.
[5] Reik W. et al. (1999) J. Nat. Genet 23: 380-382.
[6] Robertson K.D. et al. (2000) Nat Genet 25(3): 338-342.
[7] Kareta MS. et al. (2006) J Biol Chem. 281(36): 25893-25902.
[8] Gokul G., Ramakrishna G., and Khosla S. (2009) Epigenetics. 4(5): 322-329.
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