Product Name: DNMT1
Product Number: AB-NN241-3
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: DNA (cytosine-5)-methyltransferase 1
Target Alias: DNA Mtase; DNMT; MCMT; DNA methyltransferase 1; AIM; CXXC9; DNMT; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI
Product Type Specific: DNA methyltransferase pan-specific antibody
Antibody Code: NN241-3
Antibody Target Type: Pan-specific
Protein UniProt: P26358
Protein SigNET: P26358
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Target Alias: DNA Mtase; DNMT; MCMT; DNA methyltransferase 1; AIM; CXXC9; DNMT; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI
Product Type Specific: DNA methyltransferase pan-specific antibody
Antibody Code: NN241-3
Antibody Target Type: Pan-specific
Protein UniProt: P26358
Protein SigNET: P26358
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 0.05% BSA, 0.05% sodium azide, 50% glycerol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 0.05% BSA, 0.05% sodium azide, 50% glycerol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry
Antibody Dilution Recommended: WB (1:250), IHC (1:500); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~180 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: 2 µg/ml of SMC-231 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Related Product 1: DNMT1 pan-specific antibody (Cat. No.: AB-NN241-1)
Related Product 2: DNMT1 pan-specific antibody (Cat. No.: AB-NN241-2)
Antibody Potency: In mouse brain lysates, this antibody detects a ~180 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse
Antibody Positive Control: 2 µg/ml of SMC-231 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Related Product 1: DNMT1 pan-specific antibody (Cat. No.: AB-NN241-1)
Related Product 2: DNMT1 pan-specific antibody (Cat. No.: AB-NN241-2)
Scientific Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
References
[1] Bestor T. et al.(1988) J.Mol. Biol. 203: 971-983.
[2] Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl. Acids Res. 20: 2287-2291.
[3] Xie S. et al. (1999) Gene 236: 87-95.
[4] Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257.
[5] Reik W. et al. (1999) J. Nat. Genet 23: 380-382.
[6] Robertson K.D. et al. (2000) Nat Genet 25(3): 338-342.
[1] Bestor T. et al.(1988) J.Mol. Biol. 203: 971-983.
[2] Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl. Acids Res. 20: 2287-2291.
[3] Xie S. et al. (1999) Gene 236: 87-95.
[4] Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257.
[5] Reik W. et al. (1999) J. Nat. Genet 23: 380-382.
[6] Robertson K.D. et al. (2000) Nat Genet 25(3): 338-342.
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