Product Name: Cpn10
Product Number: AB-NN235-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: 10 kDa heat shock protein, mitochondrial
Target Alias: 10kDa Chaperonin; Chaperonin 10; Cpn 10; EPF; GROES; Heat Shock 10kD protein1; HSP10; HSPE1
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN235-1
Antibody Target Type: Pan-specific
Protein UniProt: P61604
Protein SigNET: P61604
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Human Cpn10 peptide AA 91-101
Target Alias: 10kDa Chaperonin; Chaperonin 10; Cpn 10; EPF; GROES; Heat Shock 10kD protein1; HSP10; HSPE1
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN235-1
Antibody Target Type: Pan-specific
Protein UniProt: P61604
Protein SigNET: P61604
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Human Cpn10 peptide AA 91-101
Production Method: Protein A purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.3, 0.02% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.3, 0.02% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Very high potency. Detects a ~ 10 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Bovine | Dog | Guinea Pig | Pig | Rabbit | Sheep | Xenopus
Antibody Positive Control: 1 µg/ml of SPC-193 was sufficient for detection of Cpn10 in 10 µg of Hela lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Species Reactivity: Human | Mouse | Rat | Bovine | Dog | Guinea Pig | Pig | Rabbit | Sheep | Xenopus
Antibody Positive Control: 1 µg/ml of SPC-193 was sufficient for detection of Cpn10 in 10 µg of Hela lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kDa (HSP10s). Cpn10 acts as a co-chaperone and interacts with the HSP60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process (1). Cpn10 has been located in human platelets, but is also present in human maternal serum (2, 3). It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy (4).
References
[1] Velez-Granell C.S. et al. (1994) J of Cell Science. 107(3): 539-549.
[2] Morton H., Hegh V., and Clunie G.J.A. (1974) Nature (London) 249: 459-460.
[3] Cavanagh A.C., and Morton H. (1994) Eur. J. Biochem. 222: 551-560.
[4] Minto M. et al. (1998) Molecular Cell Research. 1403 (2): 151-157.
[1] Velez-Granell C.S. et al. (1994) J of Cell Science. 107(3): 539-549.
[2] Morton H., Hegh V., and Clunie G.J.A. (1974) Nature (London) 249: 459-460.
[3] Cavanagh A.C., and Morton H. (1994) Eur. J. Biochem. 222: 551-560.
[4] Minto M. et al. (1998) Molecular Cell Research. 1403 (2): 151-157.
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