Product Name: Clcn3
Product Number: AB-NN234-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: H(+)/Cl(-) exchange transporter 3
Target Alias: CIC3; CLC3; CLCN 3; Chloride channel 3; Chloride channel protein 3; Chloride channel; voltage-sensitive 3; Chloride transporter ClC 3; DKFZp564I0463; Chloride transporter ClC-3; ClC-3; H(+)/Cl(-) exchange transporter 3
Product Type Specific: Chloride channel pan-specific antibody
Antibody Code: NN234-1
Antibody Target Type: Pan-specific
Protein UniProt: P51790
Protein SigNET: P51790
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Target Alias: CIC3; CLC3; CLCN 3; Chloride channel 3; Chloride channel protein 3; Chloride channel; voltage-sensitive 3; Chloride transporter ClC 3; DKFZp564I0463; Chloride transporter ClC-3; ClC-3; H(+)/Cl(-) exchange transporter 3
Product Type Specific: Chloride channel pan-specific antibody
Antibody Code: NN234-1
Antibody Target Type: Pan-specific
Protein UniProt: P51790
Protein SigNET: P51790
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~90 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-408 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).
Antibody Potency: In mouse brain lysates, this antibody detects a ~90 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-408 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).
Scientific Background: Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory.
© Kinexus Bioinformatics Corporation 2017