Product Name: CENP-A
Product Number: AB-NN231-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Histone H3-like centromeric protein A
Target Alias: CENP A; cenpa; Centromere auto antigen A; Histone H3 like centromeric protein A
Product Type Specific: Centromere protein pan-specific antibody
Antibody Code: NN231-1
Antibody Target Type: Pan-specific
Protein UniProt: P49450
Protein SigNET: P49450
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide corresponding to a portion of human CENP-A
Target Alias: CENP A; cenpa; Centromere auto antigen A; Histone H3 like centromeric protein A
Product Type Specific: Centromere protein pan-specific antibody
Antibody Code: NN231-1
Antibody Target Type: Pan-specific
Protein UniProt: P49450
Protein SigNET: P49450
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide corresponding to a portion of human CENP-A
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~18 kDa protein in cell and tissue lysates by Western blotting. Recognizes human CENP-A.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~18 kDa protein in cell and tissue lysates by Western blotting. Recognizes human CENP-A.
Antibody Species Reactivity: Human
Antibody Positive Control: 1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Positive Control: 1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Scientific Background: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.
References
[1] Rieder C.L. et al. (1998) Trends Cell Biol. 8: 310-31
[2] Choo K.H. (2000) Trends Cell Biol. 10: 182-188.
[3] Muro Y. et al. (2000) Clin. Exp. Immunol. 120: 218-223.
[4] Howman E.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1148-1153.
[1] Rieder C.L. et al. (1998) Trends Cell Biol. 8: 310-31
[2] Choo K.H. (2000) Trends Cell Biol. 10: 182-188.
[3] Muro Y. et al. (2000) Clin. Exp. Immunol. 120: 218-223.
[4] Howman E.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1148-1153.
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