Product Name: CD74
Product Number: AB-NN229-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: HLA class II histocompatibility antigen gamma chain

Target Alias: DHLAG; HLA DR gamma; HLADG; p33; p35; Protein 41

Product Type Specific: CD74 pan-specific antibody

Antibody Code: NN229-1

Antibody Target Type: Pan-specific

Protein UniProt: P04233

Protein SigNET: P04233

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG

Antibody Immunogen Source: Human CD74 invariant chain synthetic peptide

Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | ICC/Immunofluorescence | Immunoprecipitation | FCM | FACS

Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects ~33-35 kDa protein protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.

Antibody Species Reactivity: Human | Mouse

Antibody Positive Control: 1 µg/ml of SMC-116 was sufficient for detection of CD74 in 20 µg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.

Antibody Specificity: Very high

Scientific Background: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
References

[1] Becker-Hermann, S., Arie, G., Medvedovsky H, Kerem A, and Shachar I. (2005) Mol Bio Cell. 16(11):5061-9.

[2] Barrera CA., et al (2005) J Histochem Cytochem 53 (12): 1481-9.

[3] Starlets D. et al. (2006) Blood. 107 (12): 4807-4816.

[4] Burton JD. et al. (2004). Clin Cancer Res. 10(19): 6606-11.

[5] Denzin L.K., Hammond, C. and Cresswell, P. (1996) J. Exp. Med. 184: 2153-2165.

[6] Denzin L.K., Robbins N.F., Carboy-Newcomb C. and Cresswell P. (1994) Immunity 1: 595-606.