Product Name: Cav beta 4
Product Number: AB-NN223-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Voltage-dependent L-type calcium channel subunit beta-4
Target Alias: CAB4; CACNLB4; EA5; EJM; Calcium channel voltage dependent beta 4 subunit
Product Type Specific: Calcium channel pan-specific antibody
Antibody Code: NN223-1
Antibody Target Type: Pan-specific
Protein UniProt: O00305
Protein SigNET: O00305
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide amino acids 458-474 of rat CavB4
Target Alias: CAB4; CACNLB4; EA5; EJM; Calcium channel voltage dependent beta 4 subunit
Product Type Specific: Calcium channel pan-specific antibody
Antibody Code: NN223-1
Antibody Target Type: Pan-specific
Protein UniProt: O00305
Protein SigNET: O00305
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide amino acids 458-474 of rat CavB4
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium-high potency. Detects a ~50 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Hamster
Antibody Positive Control: 1 µg/ml of SMC-318 was sufficient for detection of Cavβ4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivity against rat Cab Beta1b, Cav beta2a, and Cav betas3 in transfected cells.
Related Product 1: Cav beta 1 pan-specific antibody (Cat. No.: AB-NN222-1)
Related Product 2: Cav beta 2 pan-specific antibody (Cat. No.: AB-NN228-1)
Antibody Species Reactivity: Human | Mouse | Rat | Hamster
Antibody Positive Control: 1 µg/ml of SMC-318 was sufficient for detection of Cavβ4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivity against rat Cab Beta1b, Cav beta2a, and Cav betas3 in transfected cells.
Related Product 1: Cav beta 1 pan-specific antibody (Cat. No.: AB-NN222-1)
Related Product 2: Cav beta 2 pan-specific antibody (Cat. No.: AB-NN228-1)
Related Product 3: Cav1.2 pan-specific antibody (Cat. No.: AB-NN225-1)
Related Product 4: Cav1.3 pan-specific antibody (Cat. No.: AB-NN226-1)
Related Product 5: Cav3.1 pan-specific antibody (Cat. No.: AB-NN227-1)
Related Product 4: Cav1.3 pan-specific antibody (Cat. No.: AB-NN226-1)
Related Product 5: Cav3.1 pan-specific antibody (Cat. No.: AB-NN227-1)
Scientific Background: This gene encodes a member of the beta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein described in this record plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized (1, 2).
References
[1] “Entrez Gene: CACNB4 calcium channel, voltage-dependent, beta 4 subunit”
[2] Xie M. et al. (2007) J Cell Biol. 178(3): 489-502.
[1] “Entrez Gene: CACNB4 calcium channel, voltage-dependent, beta 4 subunit”
[2] Xie M. et al. (2007) J Cell Biol. 178(3): 489-502.
© Kinexus Bioinformatics Corporation 2017