Product Name: Aquaporin 4
Product Number: AB-NN202-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Aquaporin 4
Target Alias: AQP 4; AQP-4; AQP4; AQP4_HUMAN; Aquaporin type 4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; Mercurial-insensitive water channel; MGC22454; MIWC; WCH 4; WCH4
Product Type Specific: Aquaporin pan-specific antibody
Antibody Code: NN202-1
Antibody Target Type: Pan-specific
Protein UniProt: P55087
Protein SigNET: P55087
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Target Alias: AQP 4; AQP-4; AQP4; AQP4_HUMAN; Aquaporin type 4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; Mercurial-insensitive water channel; MGC22454; MIWC; WCH 4; WCH4
Product Type Specific: Aquaporin pan-specific antibody
Antibody Code: NN202-1
Antibody Target Type: Pan-specific
Protein UniProt: P55087
Protein SigNET: P55087
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~35 kDa protein in cell and tissue lysates by Western blotting. May detect larger glycosylated ~50 kDa proteins.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 0.5 µg/ml of SPC-505 was sufficient for detection of aquaporin 4 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: Aquaporin 1 pan-specific antibody (Cat. No.: AB-NN199-1)
Related Product 2: Aquaporin 2 pan-specific antibody (Cat. No.: AB-NN200-1)
Antibody Potency: Detects a ~35 kDa protein in cell and tissue lysates by Western blotting. May detect larger glycosylated ~50 kDa proteins.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 0.5 µg/ml of SPC-505 was sufficient for detection of aquaporin 4 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: Aquaporin 1 pan-specific antibody (Cat. No.: AB-NN199-1)
Related Product 2: Aquaporin 2 pan-specific antibody (Cat. No.: AB-NN200-1)
Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 4 is constitutively expressed in the basolateral cell membrane of principal collecting duct cells in the kidney and provide a pathway for water to exit these cells (3). It is also expressed in astrocytes and is up regulated by direct insult to the CNS (4). Aquaporin 4 has been proposed as the primary autoimmune target in neuromyelitis optica ( 5, 6).
References
[1] Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.
[2] Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.
[3] Agre P., Nielsen S. (1996) Nephrologie. 17(7): 409-415.
[4] Nagelhus E.A., Mathiisen T.M., Ottersen O.P. (2004) Neuroscience. 129(4): 905-913.
[5] Lennon V.A., Kryzer T.J., Pittock S.J., Verkman A.S., Hinson S.R. (2005) J Exp Med. 202(4): 473-477.
[6] Bradl M., Lassmann D.H. (2008) Int. MS Journal/MS Forum. 15(3): 75-78.
[1] Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.
[2] Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.
[3] Agre P., Nielsen S. (1996) Nephrologie. 17(7): 409-415.
[4] Nagelhus E.A., Mathiisen T.M., Ottersen O.P. (2004) Neuroscience. 129(4): 905-913.
[5] Lennon V.A., Kryzer T.J., Pittock S.J., Verkman A.S., Hinson S.R. (2005) J Exp Med. 202(4): 473-477.
[6] Bradl M., Lassmann D.H. (2008) Int. MS Journal/MS Forum. 15(3): 75-78.
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