Product Name: Aquaporin 3
Product Number: AB-NN201-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Aquaporin 3
Target Alias: AQP3; AQP 3; AQP-3; Aqp3; AQP3_HUMAN; Aquaglyceroporin-3; Aquaporin 3 (GIL blood group); Aquaporin 3 (Gill blood group); GIL; Gill blood group
Product Type Specific: Aquaporin pan-specific antibody
Antibody Code: NN201-1
Antibody Target Type: Pan-specific
Protein UniProt: Q92482
Protein SigNET: Q92482
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Target Alias: AQP3; AQP 3; AQP-3; Aqp3; AQP3_HUMAN; Aquaglyceroporin-3; Aquaporin 3 (GIL blood group); Aquaporin 3 (Gill blood group); GIL; Gill blood group
Product Type Specific: Aquaporin pan-specific antibody
Antibody Code: NN201-1
Antibody Target Type: Pan-specific
Protein UniProt: Q92482
Protein SigNET: Q92482
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~31.5 kDa protein in cell and tissue lysates by Western blotting. May detect larger glycosylated ~35-50 kDa proteins.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 0.5 µg/ml of SPC-504 was sufficient for detection of aquaporin 3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: Aquaporin 1 pan-specific antibody (Cat. No.: AB-NN199-1)
Related Product 2: Aquaporin 2 pan-specific antibody (Cat. No.: AB-NN200-1)
Antibody Potency: Detects a ~31.5 kDa protein in cell and tissue lysates by Western blotting. May detect larger glycosylated ~35-50 kDa proteins.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 0.5 µg/ml of SPC-504 was sufficient for detection of aquaporin 3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: Aquaporin 1 pan-specific antibody (Cat. No.: AB-NN199-1)
Related Product 2: Aquaporin 2 pan-specific antibody (Cat. No.: AB-NN200-1)
Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 3 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells (3). AQP3 gene expression is not regulated by vasopressin (4).
References
[1] Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.
[2] Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.
[3] Sasaki S., Ishibashi K., and Marumo F. (1998) Annu Rev Physiol. 60: 199-220.
[4] Dibas A.I., Mia A.J., Yorio T. (1998) Proc Soc Exp Med. 219(3): 183-199.
[1] Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.
[2] Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.
[3] Sasaki S., Ishibashi K., and Marumo F. (1998) Annu Rev Physiol. 60: 199-220.
[4] Dibas A.I., Mia A.J., Yorio T. (1998) Proc Soc Exp Med. 219(3): 183-199.
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