Product Name: Aquaporin 2
Product Number: AB-NN200-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Aquaporin 2
Target Alias: ADH water channel; AQP 2; AQP CD; AQP-2; AQP-CD; AQP2; AQP2_HUMAN; AQPCD; Aquaporin 2 collecting duct; Aquaporin CD; Aquaporin2; Aquaporine 2; Collecting duct water channel protein; MGC34501; Water channel aquaporin 2; Water channel protein for renal collecting duct; WCH CD; WCH-CD; WCHCD
Product Type Specific: Aquaporin pan-specific antibody
Antibody Code: NN200-1
Antibody Target Type: Pan-specific
Protein UniProt: P41181
Protein SigNET: P41181
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Target Alias: ADH water channel; AQP 2; AQP CD; AQP-2; AQP-CD; AQP2; AQP2_HUMAN; AQPCD; Aquaporin 2 collecting duct; Aquaporin CD; Aquaporin2; Aquaporine 2; Collecting duct water channel protein; MGC34501; Water channel aquaporin 2; Water channel protein for renal collecting duct; WCH CD; WCH-CD; WCHCD
Product Type Specific: Aquaporin pan-specific antibody
Antibody Code: NN200-1
Antibody Target Type: Pan-specific
Protein UniProt: P41181
Protein SigNET: P41181
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~28.8 kDa protein in cell and tissue lysates by Western blotting. May detect larger glycosylated ~35-50 kDa proteins.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 0.5 µg/ml of SPC-503 was sufficient for detection of aquaporin 2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: Aquaporin 1 pan-specific antibody (Cat. No.: AB-NN199-1)
Related Product 2: Aquaporin 3 pan-specific antibody (Cat. No.: AB-NN201-1)
Antibody Potency: Detects a ~28.8 kDa protein in cell and tissue lysates by Western blotting. May detect larger glycosylated ~35-50 kDa proteins.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 0.5 µg/ml of SPC-503 was sufficient for detection of aquaporin 2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: Aquaporin 1 pan-specific antibody (Cat. No.: AB-NN199-1)
Related Product 2: Aquaporin 3 pan-specific antibody (Cat. No.: AB-NN201-1)
Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4).
References
[1] Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.
[2] Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.
[3] Lodish H.F. (2008) Molecular Cell Biology. New York: W.H. Freeman. Print. 445.
[4] www.vivo.colostate.edu/hbooks/molecules/aquaporins.html
[1] Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.
[2] Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.
[3] Lodish H.F. (2008) Molecular Cell Biology. New York: W.H. Freeman. Print. 445.
[4] www.vivo.colostate.edu/hbooks/molecules/aquaporins.html
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