Product Name: Aquaporin 1
Product Number: AB-NN199-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Aquaporin 1
Target Alias: AQP1; AQP 1; AQP CHIP; AQP-1; AQP1; AQP1_HUMAN; Aquaporin CHIP; Aquaporin-CHIP; Aquaporin1; Channel forming integral protein 28kDa; Channel like integral membrane protein 28 kDa; CHIP 28; CHIP28; CO; Colton blood group; Growth factor induced delayed early response protein; MGC26324; Urine water channel; Water channel protein CHIP 29; Water channel protein CHIP29; Water channel protein for red blood cells and kidney proximal tubule
Product Type Specific: Aquaporin pan-specific antibody
Antibody Code: NN199-1
Antibody Target Type: Pan-specific
Protein UniProt: P29972
Protein SigNET: P29972
Antibody Type: Polyclonal
Target Alias: AQP1; AQP 1; AQP CHIP; AQP-1; AQP1; AQP1_HUMAN; Aquaporin CHIP; Aquaporin-CHIP; Aquaporin1; Channel forming integral protein 28kDa; Channel like integral membrane protein 28 kDa; CHIP 28; CHIP28; CO; Colton blood group; Growth factor induced delayed early response protein; MGC26324; Urine water channel; Water channel protein CHIP 29; Water channel protein CHIP29; Water channel protein for red blood cells and kidney proximal tubule
Product Type Specific: Aquaporin pan-specific antibody
Antibody Code: NN199-1
Antibody Target Type: Pan-specific
Protein UniProt: P29972
Protein SigNET: P29972
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Produced against the N-terminal peptide (sequence N-MASEFKKKLF) of rat Aquaporin 1
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Produced against the N-terminal peptide (sequence N-MASEFKKKLF) of rat Aquaporin 1
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~28.5 kDa protein in cell and tissue lysates by Western blotting. May detect larger glycosylated ~35-50 kDa proteins.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 0.5 µg/ml of SPC-502 was sufficient for detection of aquaporin 1 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: Aquaporin 2 pan-specific antibody (Cat. No.: AB-NN200-1)
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~28.5 kDa protein in cell and tissue lysates by Western blotting. May detect larger glycosylated ~35-50 kDa proteins.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 0.5 µg/ml of SPC-502 was sufficient for detection of aquaporin 1 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: Aquaporin 2 pan-specific antibody (Cat. No.: AB-NN200-1)
Related Product 2: Aquaporin 3 pan-specific antibody (Cat. No.: AB-NN201-1)
Related Product 3: Aquaporin 4 pan-specific antibody (Cat. No.: AB-NN202-1)
Related Product 3: Aquaporin 4 pan-specific antibody (Cat. No.: AB-NN202-1)
Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquarporin 1 is a widely expressed water channel, found in the basolateral and apical plasma membranes of the proximal tubes, the descending loop of Henel and in the descending portion of the vasa recta. Additionally it is found in red blood cells, vascular endothelium, gastrointestinal tract, sweat glands and lungs. It is not regulated by vasopressin (3).
References
[1] Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.
[2] Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.
[3] www.vivo.colostate.edu/hbooks/molecules/aquaporins.html
[1] Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.
[2] Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.
[3] www.vivo.colostate.edu/hbooks/molecules/aquaporins.html
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