Product Name: AHA2
Product Number: AB-NN192-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Activator of 90 kDa heat shock protein ATPase homologue 2
Target Alias: ATPase hydrogen-exporting ATPase 2
Product Type Specific: AHA2 pan-specific antibody
Antibody Code: NN192-1
Antibody Target Type: Pan-specific
Protein UniProt: Q719I0
Protein SigNET: Q719I0
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Aha2
Production Method: Protein A purified
Target Alias: ATPase hydrogen-exporting ATPase 2
Product Type Specific: AHA2 pan-specific antibody
Antibody Code: NN192-1
Antibody Target Type: Pan-specific
Protein UniProt: Q719I0
Protein SigNET: Q719I0
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Aha2
Production Method: Protein A purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:80); optimal dilutions for assays should be determined by the user.
Antibody Potency: Cross reacts with Aha1.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:80); optimal dilutions for assays should be determined by the user.
Antibody Potency: Cross reacts with Aha1.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SPC-196 was sufficient for detection of Aha2 in 20 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Antibody Specificity: Medium
Antibody Cross Reactivity: Antibody seems to have a generally high background in mouse brain lysates.
Antibody Positive Control: 1 µg/ml of SPC-196 was sufficient for detection of Aha2 in 20 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Antibody Specificity: Medium
Antibody Cross Reactivity: Antibody seems to have a generally high background in mouse brain lysates.
Scientific Background: The Arabidopsis protein RIN4 is a well known regulator of plant immunity. The plasma membrane H+-ATPases Aha1 and Aha2 are one class of RIN4-associated proteins (1-5). Aha1 and Aha2 play a crucial role in resisting pathogen invasion- plants use RIN4 to regulate H+-ATPase activity during immune responses, thereby controlling stomatal apertures during pathogen attack (1). Wild type AHA2 has been found to be localized to the plasma membrane, and has also been found in the ER (4).
References
[1] Liu J., Elmore J.M., and Coaker G. (2009) Plant Signal Behav. 4(12): 1107-1110.
[2] Harper J.F., Surowy T.K. and Sussman M.R. (1989) Proc Natl Acad. Sci USA . 86: 1234-1238.
[3] Harper J. F. et al. (1990) J Biol Chem. 265: 13601-13608.
[4] Harper J.F., Manney L., and Sussman M.R. (1994) Mol Gen Genet. 243: 572-587.
[5] Regenberg B., Villalba J.M., Lanfermeijer F.C., and Palmgren M.G. (1995) Plant Cell. 7: 1655-1666.
[1] Liu J., Elmore J.M., and Coaker G. (2009) Plant Signal Behav. 4(12): 1107-1110.
[2] Harper J.F., Surowy T.K. and Sussman M.R. (1989) Proc Natl Acad. Sci USA . 86: 1234-1238.
[3] Harper J. F. et al. (1990) J Biol Chem. 265: 13601-13608.
[4] Harper J.F., Manney L., and Sussman M.R. (1994) Mol Gen Genet. 243: 572-587.
[5] Regenberg B., Villalba J.M., Lanfermeijer F.C., and Palmgren M.G. (1995) Plant Cell. 7: 1655-1666.
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