Product Name: PDK3-3
Product Number: AB-NN181-2
Size: 25 µg      Price:89.00
      $US
Target Full Name: Pyruvate dehydrogenase kinase isoform 3; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial

Target Alias: Kinase Pyruvate dehydrogenase kinase 3; PDK3

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NN181-2

Antibody Target Type: Pan-specific

Protein UniProt: Q15120

Protein SigNET: Q15120

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PDK3 (PDHK3) sequence peptide Cat. No.: PE-01BAE99

Antibody Immunogen Sequence: LEYENKSPEDPQVLDC

Antibody Immunogen Description: Corresponds to amino acid residues L92 to D106

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Weak reactivity with recombinant human PDK3 on protein dot blots.

Antibody Species Reactivity: Human; Mouse

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 42-53 kDa.

Antibody Specificity: Medium

Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human PDK1, PDK2 and PDK4

Related Product 1: PDK3-3 blocking peptide

Related Product 2: PDK3-1 pan-specific antibody (Cat. No.: AB-NN181-1)

Related Product 3: PDHKSubtide - PDHK protein kinase substrate peptide

Scientific Background: PDHK3 (PDK3) is a protein-serine/threonine kinase of the Atypical group and PDHK family. It phosphorylates and inactivates the enzyme pyruvate dehydrogenase. It is mostly expressed in the heart and skeletal muscles, and it regulates glucose metabolism and maintenance of glucose homeostasis under starvation. Inactivation of PDHK3 signalling drives melanomas towards oxidative metabolism and allows for the possibility of using pro-oxidants as therapies. PDHK3 has been shown to be overexpressed in colon cancer cell lines.
Figure 1. Dot blotting PDK3-3 antibody with recombinant purified proteins.