Product Name: PDK1-2
Product Number: AB-NN179-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: Pyruvate dehydrogenase kinase isoform 1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial

Target Alias: Kinase Pyruvate dehydrogenase kinase 1; Pyruvate dehydrogenase [lipoamide] kinase isozyme 1, mitochondrial precursor; Pyruvate dehydrogenase kinase isoform 1; Pyruvate dehydrogenase kinase, isozyme 1; PDK1

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NN179-1

Antibody Target Type: Pan-specific

Protein UniProt: Q15118

Protein SigNET: Q15118

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PDK1 (PDHK1) sequence peptide Cat. No.: PE-01AZX90

Antibody Immunogen Sequence: CNVLEVIKDGYENARR

Antibody Immunogen Description: Corresponds to amino acid residues C223 to R238

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 1 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with recombinant human PDK1 on protein dot blots.

Antibody Species Reactivity: Human; Mouse

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-65 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human PDK2, PDK3 and PDK4.

Related Product 1: PDK1-2 blocking peptide

Related Product 2: PDK1-3 pan-specific antibody (Cat. No.: AB-NN179-2)

Related Product 3: PDHKSubtide - PDHK protein kinase substrate peptide

Scientific Background: PDHK1 (PDK1, pyruvate dehydrogenase kinase 1) is a protein-serine/threonine kinase of the Atypical group and PDHK family. It functions in the regulation of glucose and fatty acid metabolism through the phosphorylation of the subunits PDHA1 and PDHA2 of pyruvate dehydrogenase. This inhibitory phosphorylation reduces metabolite flux into the trichloroacetic acid (TCA) cycle, decreases aerobic respiration, and prevents the formation of acetyl-CoA. In addition, PDHK1 functions to prevent apoptosis in cells exposed to hypoxia or oxidative stress. Due to the presence of HIF-1, PDHK1 has shown to have increased activity in hypoxic cancer cells. PDHK1 shunts pyruvate away from the citric acid cycle and helps to maintain hypoxic cell viability. PDHK1 may be a target for antitumour therapy, since PDK1 prevents apoptosis in these cancerous cells.
Figure 1. Dot blotting PDK1-2 antibody with recombinant purified proteins.