Product Name: PDK4-1
Product Number: AB-NN178-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Pyruvate dehydrogenase kinase isoform 4; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
Target Alias: Pyruvate dehydrogenase kinase isoform 4; PDK4;
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NN178-2
Antibody Target Type: Pan-specific
Protein UniProt: Q16654
Protein SigNET: Q16654
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PDK4 (PDHK4) sequence peptide Cat. No.: PE-01ARI99
Target Alias: Pyruvate dehydrogenase kinase isoform 4; PDK4;
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NN178-2
Antibody Target Type: Pan-specific
Protein UniProt: Q16654
Protein SigNET: Q16654
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PDK4 (PDHK4) sequence peptide Cat. No.: PE-01ARI99
Antibody Immunogen Sequence: CREVEHFSRYSPSPLS
Antibody Immunogen Description: Corresponds to amino acid residues R22 to S36
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues R22 to S36
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human PDK4 on protein dot blots.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human PDK4 on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-55 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human PDK1, PDK3 and PDK4.
Related Product 1: PDK4-1 blocking peptide
Related Product 2: PDK4 pan-specific antibody (Cat. No.: AB-NN178-1)
Related Product 3: PDK4 pan-specific antibody (Cat. No.: AB-NN178-2P)
Related Product 4: PDK4 pan-specific antibody (Cat. No.: AB-NN178-3P)
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-55 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human PDK1, PDK3 and PDK4.
Related Product 1: PDK4-1 blocking peptide
Related Product 2: PDK4 pan-specific antibody (Cat. No.: AB-NN178-1)
Related Product 3: PDK4 pan-specific antibody (Cat. No.: AB-NN178-2P)
Related Product 4: PDK4 pan-specific antibody (Cat. No.: AB-NN178-3P)
Related Product 5: PDK4-3 pan-specific antibody (Cat. No.: AB-NN178-3)
Related Product 6: PDHKSubtide - PDHK protein kinase substrate peptide
Related Product 6: PDHKSubtide - PDHK protein kinase substrate peptide
Scientific Background: PDHK4 (PDK4, pyruvate dehydrogenase kinase 4) is a protein-serine/threonine kinase of the Atypical group and PDHK family. It phosphorylates and inactivates the enzyme pyruvate dehydrogenase. Deficiency of PDHK4 attenuates the long-term negative effects of a high-saturated fat diet.
Figure 1. Dot blotting PDK4-1 antibody with recombinant purified proteins.
Figure 2. Immunoblotting of various cell lines with NN178-2 antibody at a 500 X dilution. The target protein PDK4 is indicated. The expected molecular mass of PDK4 is 46,469 Da. Each lane was loaded with 20 µg of cell lysate protein.
© Kinexus Bioinformatics Corporation 2017