Product Name: alpha B Crystallin
Product Number: AB-NN149-1
| Size: | 50 µl | Price: | 89.00 | |
| $US |
Target Full Name: Alpha-crystallin B chain
Target Alias: AACRYA; CRYA2; CRYAB; CTPP2; HspB5; NY REN 27 antigen
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN149-1
Antibody Target Type: Pan-specific
Protein UniProt: P02511
Protein SigNET: P02511
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide corresponding to human alpha B crystallin conjugated to KLH.
Production Method: Rabbit antiserum
Target Alias: AACRYA; CRYA2; CRYAB; CTPP2; HspB5; NY REN 27 antigen
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN149-1
Antibody Target Type: Pan-specific
Protein UniProt: P02511
Protein SigNET: P02511
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide corresponding to human alpha B crystallin conjugated to KLH.
Production Method: Rabbit antiserum
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Storage Buffer: Rabbit antiserum
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting, Immunohistochemistry, ICC
Antibody Dilution Recommended: 1:5000-10000 (ECL) (WB)
Antibody Potency: Very high potency. Detects a ~22 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human, Mouse, Rat, Cow, Chicken
Storage Buffer: Rabbit antiserum
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting, Immunohistochemistry, ICC
Antibody Dilution Recommended: 1:5000-10000 (ECL) (WB)
Antibody Potency: Very high potency. Detects a ~22 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human, Mouse, Rat, Cow, Chicken
Antibody Positive Control: A 1:5000 dilution of SPC-126 was sufficient for detection of alpha B crystallin in 20μg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with αA-crystallin.
Related Product 1: Alpha B Crystallin pan-specific antibody (Cat. No.: AB-NN149-2)
Related Product 2: Alpha B Crystallin pan-specific antibody (Cat. No.: AB-NN149-3)
Related Product 3: Alpha A Crystallin pan-specific antibody (Cat. No.: AB-NN149-5)
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with αA-crystallin.
Related Product 1: Alpha B Crystallin pan-specific antibody (Cat. No.: AB-NN149-2)
Related Product 2: Alpha B Crystallin pan-specific antibody (Cat. No.: AB-NN149-3)
Related Product 3: Alpha A Crystallin pan-specific antibody (Cat. No.: AB-NN149-5)
Scientific Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its’ phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).
References
[1] Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052.
[2] Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453.
[3] Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490.
[4] Horwitz J. (2003) Exp Eye Res. 76: 145-153.
[5] Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924.
[6] Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377.
[7] Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203- 215.
[8] Yaung J. et al. (2007) Molecular Vision 13: 566-577.
[1] Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052.
[2] Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453.
[3] Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490.
[4] Horwitz J. (2003) Exp Eye Res. 76: 145-153.
[5] Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924.
[6] Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377.
[7] Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203- 215.
[8] Yaung J. et al. (2007) Molecular Vision 13: 566-577.
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