Product Name: PSD95
Product Number: AB-NN142
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Disks large homologue 4
Target Alias: PSD 95; PSD-95; DLG4; SAP90; Synapse-associated protein 90; Postsynaptic density protein 95
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN142
Antibody Target Type: Pan-specific
Protein UniProt: P78352
Protein SigNET: P78352
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Recombinant rat PSD-95
Target Alias: PSD 95; PSD-95; DLG4; SAP90; Synapse-associated protein 90; Postsynaptic density protein 95
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN142
Antibody Target Type: Pan-specific
Protein UniProt: P78352
Protein SigNET: P78352
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Recombinant rat PSD-95
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:250), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:250), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~100 kDa protein in cell and tissue lysates by Western blotting. An additional protein of >100 kDa protein is also detected.
Antibody Species Reactivity: Mouse | Rat | Bovine
Antibody Positive Control: 1 µg/ml was sufficient for detection of PSD-95 on 20 µg rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse (No Suggestions) as the secondary.
Antibody Specificity: High-medium
Antibody Cross Reactivity: Some cross-reactive proteins are detected at ~75 kDa protein and 50 kDa protein in rat and mouse samples.
Related Product 1: PSD95 pan-specific antibody (Cat. No.: AB-NN142-2)
Antibody Species Reactivity: Mouse | Rat | Bovine
Antibody Positive Control: 1 µg/ml was sufficient for detection of PSD-95 on 20 µg rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse (No Suggestions) as the secondary.
Antibody Specificity: High-medium
Antibody Cross Reactivity: Some cross-reactive proteins are detected at ~75 kDa protein and 50 kDa protein in rat and mouse samples.
Related Product 1: PSD95 pan-specific antibody (Cat. No.: AB-NN142-2)
Scientific Background: Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4).
PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).
References
[1] Chetkovich D.M., Bunn R.C., Kuo S.H., Kawasaki Y., Kohwi M., and Bredt D.S. (2002) J Neurosci. 22(15): 6415-25.
[2] Cao J., Viholainen J.I., Dart C., Warwick H.K., Levland M.L. and Courtney M.J. (2005) J Cell Biol. 168(1): 117-26.
[3] Kennedy M. (1997) Trends in Neurosci. 6: 264-268.
[4] Irie M. et al. (1997) Science 277(5331): 1511-5.
[5] Cai C. et al. (2006) J Biol Chem. 281: 4267-73.
[6] Yao W.D. et al. (2004) Neuron 41: 625-38.
[7] Cline H. (2005) Curr Biol. 15: R203-5.
[1] Chetkovich D.M., Bunn R.C., Kuo S.H., Kawasaki Y., Kohwi M., and Bredt D.S. (2002) J Neurosci. 22(15): 6415-25.
[2] Cao J., Viholainen J.I., Dart C., Warwick H.K., Levland M.L. and Courtney M.J. (2005) J Cell Biol. 168(1): 117-26.
[3] Kennedy M. (1997) Trends in Neurosci. 6: 264-268.
[4] Irie M. et al. (1997) Science 277(5331): 1511-5.
[5] Cai C. et al. (2006) J Biol Chem. 281: 4267-73.
[6] Yao W.D. et al. (2004) Neuron 41: 625-38.
[7] Cline H. (2005) Curr Biol. 15: R203-5.
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