Product Name: PDI
Product Number: AB-NN141-1
Size: 25 µl      Price:89.00
      $US
Target Full Name: Protein disulfide-isomerase

Target Alias: PDA2; PDI; PDIA2; PDIP; pancreatic protein disulfide isomerase

Product Type Specific: Protein disulfide isomerase pan-specific antibody

Antibody Code: NN141-1

Antibody Target Type: Pan-specific

Protein UniProt: P07237

Protein SigNET: P07237

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Rat PDI synthetic peptide conjugated to KLH

Production Method: Rabbit antiserum

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Storage Buffer: Rabbit antiserum

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting, Immunoprecipitation, ICC, Immunohistochemistry

Antibody Dilution Recommended: 1:4000 (WB)

Antibody Potency: Very high potency. Detects a ~58 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human, Mouse, Rat, Dog, Hamster, Monkey, Guinea pig, Bovine, Sheep, Pig, Xenopus, Perna viridis (Mussel))
Antibody Positive Control: A 1:4000 dilution of SPC-114 was sufficient for detection of PDI in 20μg of HeLa cell lysate by ECL immunoblot analysis.

Antibody Specificity: Very high

Scientific Background: The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).
References

[1] NA K.S. et al. (2007) Mol Cells. 24(2): 261-7.

[2] Mayer M., Kies U., Kammermeier R., and Buchner J. (2000) J Biol Chem. 275(38): 29421-5.

[3] Delom F., Mallet B., Carayon P., and Lejeune P.J. (2001) J Biol Chem 276(24): 21337-42.

[4] Schultz-Norton J.R., McDonald W.H., Yates J.R. and Nardulli A.M. (2006) Mol Endocrinol 20(9): 1982-95.

[5] Turano C., Coppari S. Altieri F. and Ferraro (2002) J Cell Physiol 193: 154-163.

[6] Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819.

[7] Yoshimori T. et al. (1990) J Biol Chem. 265(26): 15984-90.