Product Name: Calnexin-CT
Product Number: AB-NN136-4
| Size: | 50 µl | Price: | 89.00 | |
| $US |
Target Full Name: Calnexin
Target Alias: CALX_HUMAN; CANX; CNX; FLJ26570; Histocompatibility complex class I antigen binding protein p88; IP90; Major histocompatibility complex class I antigen-binding protein p88
Product Type Specific: Calcium binding protein pan-specific antibody
Antibody Code: NN136-4
Antibody Target Type: Pan-specific
Protein UniProt: P27824
Protein SigNET: P27824
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: CALX_HUMAN; CANX; CNX; FLJ26570; Histocompatibility complex class I antigen binding protein p88; IP90; Major histocompatibility complex class I antigen-binding protein p88
Product Type Specific: Calcium binding protein pan-specific antibody
Antibody Code: NN136-4
Antibody Target Type: Pan-specific
Protein UniProt: P27824
Protein SigNET: P27824
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Production Method: Rabbit antiserum
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Storage Buffer: Rabbit antiserum
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting, Immunohistochemistry, ICC, Immunoprecipitation, Flow cytometry, Immunofluorescence
Production Method: Rabbit antiserum
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Storage Buffer: Rabbit antiserum
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting, Immunohistochemistry, ICC, Immunoprecipitation, Flow cytometry, Immunofluorescence
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Very high potency. Detects a ~90 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human, Monkey, Mouse, Rat, Bovine, Chicken (weak), Dog, Guinea pig, Hamster, Pig, Quail, Rabbit, Sheep, Drosophila (weak), Xenopus (weak)
Antibody Positive Control: A 1:1000 dilution of SPC-108 was sufficient for detection of Calnexin in 10μg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Specificity: Very high
Related Product 1: Calnexin-CT pan-specific antibody (Cat. No.: AB-NN136-5)
Related Product 2: Calnexin-NT pan-specific antibody (Cat. No.: AB-NN136-2)
Antibody Potency: Very high potency. Detects a ~90 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human, Monkey, Mouse, Rat, Bovine, Chicken (weak), Dog, Guinea pig, Hamster, Pig, Quail, Rabbit, Sheep, Drosophila (weak), Xenopus (weak)
Antibody Positive Control: A 1:1000 dilution of SPC-108 was sufficient for detection of Calnexin in 10μg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Specificity: Very high
Related Product 1: Calnexin-CT pan-specific antibody (Cat. No.: AB-NN136-5)
Related Product 2: Calnexin-NT pan-specific antibody (Cat. No.: AB-NN136-2)
Scientific Background: Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulitsubunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
References
[1] Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90.
[2] Tjoelker L.W. et al. (1994) Biochemistry 33: 3229.
[3] Schrag J. et al. (2001) Molecular Cell 8(3): 633-644.
[4] Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819.
[5] Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460.
[6] Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97- 103.
[7] Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
[1] Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90.
[2] Tjoelker L.W. et al. (1994) Biochemistry 33: 3229.
[3] Schrag J. et al. (2001) Molecular Cell 8(3): 633-644.
[4] Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819.
[5] Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460.
[6] Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97- 103.
[7] Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
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