Product Name: SOD1 (UβB)
Product Number: AB-NN098-5
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Superoxide dismutase [Cu-Zn]
Target Alias: Superoxide dismutase 1; SOD; SOD1 unfolded beta barrel region
Product Type Specific: Superoxide dismutase pan-specific antibody
Antibody Code: NN098-5
Antibody Target Type: Pan-specific
Protein UniProt: P00441
Protein SigNET: P00441
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: N-terminal region of SOD1, unfolded beta barrel region
Target Alias: Superoxide dismutase 1; SOD; SOD1 unfolded beta barrel region
Product Type Specific: Superoxide dismutase pan-specific antibody
Antibody Code: NN098-5
Antibody Target Type: Pan-specific
Protein UniProt: P00441
Protein SigNET: P00441
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: N-terminal region of SOD1, unfolded beta barrel region
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Recognizes a conformation specific epitope where the beta barrel is unfolded.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1µg/ml of SPC-205 was sufficient for detection of the unfolded beta barrel of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: SOD1 (EDI) pan-specific antibody (Cat. No.: AB-NN098-4)
Related Product 2: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-2)
Related Product 3: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-1)
Related Product 4: SOD (Mn) pan-specific antibody (Cat. No.: AB-NN068-1)
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1µg/ml of SPC-205 was sufficient for detection of the unfolded beta barrel of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: SOD1 (EDI) pan-specific antibody (Cat. No.: AB-NN098-4)
Related Product 2: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-2)
Related Product 3: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-1)
Related Product 4: SOD (Mn) pan-specific antibody (Cat. No.: AB-NN068-1)
Related Product 5: SOD (Mn) pan-specific antibody (Cat. No.: AB-NN068-2)
Related Product 6: SOD (Cu/Zn) pan-specific antibody (Cat. No.: AB-NN098-3)
Related Product 7: SOD (Cu/Zn) pan-specific antibody (Cat. No.: AB-NN098-2)
Related Product 6: SOD (Cu/Zn) pan-specific antibody (Cat. No.: AB-NN098-3)
Related Product 7: SOD (Cu/Zn) pan-specific antibody (Cat. No.: AB-NN098-2)
Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as Anti-SOD1 (UβB), which targets an unfolded region of the beta barrel of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with Anti-SOD1 (EDI) (SPC-206D) which detects an exposed dimer interface (EDI) of SOD1.
References
[1] Barrister J.V. et al. (1987). Crit. Rev. Biochem. 22:111-180.
[2] Furukawa Y., O’Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6.
[3] Gao B. et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925.
[4] Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385.
[5] Kerman A. et al. (2010) Acta Neuropathol. 119:335-344.
[1] Barrister J.V. et al. (1987). Crit. Rev. Biochem. 22:111-180.
[2] Furukawa Y., O’Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6.
[3] Gao B. et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925.
[4] Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385.
[5] Kerman A. et al. (2010) Acta Neuropathol. 119:335-344.
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