Product Name: SOD (Mn)

Product Number: AB-NN068-1

Size:	25 µg		Price:	89.00
			$US

Target Full Name: Superoxide dismutase [Mn], mitochondrial

Target Alias: Manganese SOD; IPO B; Mn SOD; SOD2

Product Type Specific: Superoxide dismutase pan-specific antibody

Antibody Code: NN068-1

Antibody Target Type: Pan-specific

Protein UniProt: P04179

Protein SigNET: P04179

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Ig Isotype Clone: N/A

Antibody Immunogen Source: Rat Mn SOD

Production Method: Affinity (antigen) purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA

Antibody Dilution Recommended: WB (1:5000), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a ~25 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human | Rat | Mouse | Bovine | Dog | Chicken | Dosophila | Guinea Pig | Pig | Hamster | Monkey | Rabbit | Sheep | Xenopus | Squirrel | Ciona intestinalis (Sea squirt)

Antibody Positive Control: 0.5 µg/ml of SPC-117 was sufficient for detection of Mn SOD in 20 µg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

Related Product 1: SOD (Mn) pan-specific antibody (Cat. No.: AB-NN068-2)

Related Product 2: SOD (Cu/Zn) pan-specific antibody (Cat. No.: AB-NN098-2)

Related Product 3: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-1)

Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

Figure 1. Immunoblotting of various cell lines with AB-NN068-1 antibody at a 1000 X dilution. The target protein SOD (Mn) is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 34012.

Figure 2. Immunoblotting of various tissue lines with AB-NN068-1 antibody at a 1000 X dilution. The target protein SOD (Mn) is indicated. Each lane was loaded with 15 µg of tissue lysate protein. The max signal count was 50978.

References

[1] Adachi T. et al. (1992). Clin. Chim. Acta. 212: 89-102.

[2] Barrister J.V. et al. (1987). Crit. Rev. Biochem. 22:111-180.

[3] Furukawa Y., O’Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6.

[4] Gao B. et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925.

[5] Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385.

[6] Kurobe N. et al. (1990) Biomedical Research. 11: 187-194

[7] Wispe J.R. et al. (1989) BBA. 994: 30-36.

[8] Xiao-Hong Liu. et al. (1993) Brain Research. 625: 29-37.