Product Name: HSP90
Product Number: AB-NN061-4
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Heat shock protein HSP 90-alpha
Target Alias: HSP86; HSP89A; HSP90A; HSP90AA1; HSPC1; HSPCA; HsoCAL3
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN061-4
Antibody Target Type: Pan-specific
Protein UniProt: P07900
Protein SigNET: P07900
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Full length protein HSP90 purified from chicken brain
Production Method: Protein G purified
Target Alias: HSP86; HSP89A; HSP90A; HSP90AA1; HSPC1; HSPCA; HsoCAL3
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN061-4
Antibody Target Type: Pan-specific
Protein UniProt: P07900
Protein SigNET: P07900
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Full length protein HSP90 purified from chicken brain
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:500), IP (5µg) ; optimal dilutions for assays should be determined by the user.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:500), IP (5µg) ; optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~90 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Bovine | Pig | Rabbit | Chicken
Antibody Positive Control: 2 µg/ml was sufficient for detection of HSP90α in 20 µg of heat shocked HeLa cell lysate as well as in 100ng of human HSP90α protein by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Related Product 1: HSP90 complex pan-specific antibody (Cat. No.: AB-NN061-18)
Related Product 2: HSP90 alpha pan-specific antibody (Cat. No.: AB-NN061-19)
Related Product 3: HSP90 alpha/beta pan-specific antibody (Cat. No.: AB-NN061-2)
Antibody Species Reactivity: Human | Mouse | Rat | Bovine | Pig | Rabbit | Chicken
Antibody Positive Control: 2 µg/ml was sufficient for detection of HSP90α in 20 µg of heat shocked HeLa cell lysate as well as in 100ng of human HSP90α protein by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Related Product 1: HSP90 complex pan-specific antibody (Cat. No.: AB-NN061-18)
Related Product 2: HSP90 alpha pan-specific antibody (Cat. No.: AB-NN061-19)
Related Product 3: HSP90 alpha/beta pan-specific antibody (Cat. No.: AB-NN061-2)
Related Product 4: HSP90 pan-specific antibody (Cat. No.: AB-NN061-3)
Related Product 5: HSP90 (total) pan-specific antibody (Cat. No.: AB-NN061-9)
Related Product 6: HSP90 alpha pan-specific antibody (Cat. No.: AB-NN164)
Related Product 7: HSP90 beta pan-specific antibody (Cat. No.: AB-NN165)
Related Product 8: Hsp90 beta pan-specific antibody (Cat. No.: AB-NN165-1)
Related Product 9: HSP90AB1-pY484 phosphosite-specific antibody (Cat. No.: AB-PN520)
Related Product 10: HSP90AB1-pY484 phosphosite-specific antibody (Cat. No.: AB-PN520)
Related Product 5: HSP90 (total) pan-specific antibody (Cat. No.: AB-NN061-9)
Related Product 6: HSP90 alpha pan-specific antibody (Cat. No.: AB-NN164)
Related Product 7: HSP90 beta pan-specific antibody (Cat. No.: AB-NN165)
Related Product 8: Hsp90 beta pan-specific antibody (Cat. No.: AB-NN165-1)
Related Product 9: HSP90AB1-pY484 phosphosite-specific antibody (Cat. No.: AB-PN520)
Related Product 10: HSP90AB1-pY484 phosphosite-specific antibody (Cat. No.: AB-PN520)
Scientific Background: HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (4-7). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90- regulated proteins that have been discovered to date are involved in cell signaling (8-9). The number of proteins now known to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase(6). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immune-adsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (10). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.
References
[1] Schuh S. et al. (1985) J Biol Chem. 260 (26): 14292-14296.
[2] Lipsich L.A., Cutt J.R. and Brugge J.S. (1982) Mol. Cell Biol. 2(7): 875-880.
[3] Brugge J.S., Yonemoto W., and Darrow D. (1983) Mol. Cell. Biol. 3(1): 9-19.
[4] Arlander SJH et al. (2003) J Biol Chem 278: 52572-52577.
[5] Pearl H et al. (2001) Adv Protein Chem 59: 157-186.
[6] Neckers L et al. (2002) Trends Mol Med 8:S55-S61.
[7] Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.
[8] Pratt W, Toft D. (1997) Endocr Rev 18: 306–360.
[1] Schuh S. et al. (1985) J Biol Chem. 260 (26): 14292-14296.
[2] Lipsich L.A., Cutt J.R. and Brugge J.S. (1982) Mol. Cell Biol. 2(7): 875-880.
[3] Brugge J.S., Yonemoto W., and Darrow D. (1983) Mol. Cell. Biol. 3(1): 9-19.
[4] Arlander SJH et al. (2003) J Biol Chem 278: 52572-52577.
[5] Pearl H et al. (2001) Adv Protein Chem 59: 157-186.
[6] Neckers L et al. (2002) Trends Mol Med 8:S55-S61.
[7] Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.
[8] Pratt W, Toft D. (1997) Endocr Rev 18: 306–360.
[9] Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434.
[10] Whitesell L et al. (1994) Proc Natl Acad Sci USA 91: 8324– 8328.
[10] Whitesell L et al. (1994) Proc Natl Acad Sci USA 91: 8324– 8328.
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