Product Name: HSP47
Product Number: AB-NN058-2
| Size: | 50 µg | Price: | 89.00 | |
| $US |
Target Full Name: Serpin H1
Target Alias: Colligin; Gp46; serine proteinase inhibitor; cysteine proteinase inhibitor; collagen binding protein
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN058-2
Antibody Target Type: Pan-specific
Protein UniProt: P50454.2
Protein SigNET: P50454.2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1Kappa
Antibody Immunogen Source: Human HSP47, full length
Production Method: Protein G purified
Target Alias: Colligin; Gp46; serine proteinase inhibitor; cysteine proteinase inhibitor; collagen binding protein
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN058-2
Antibody Target Type: Pan-specific
Protein UniProt: P50454.2
Protein SigNET: P50454.2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1Kappa
Antibody Immunogen Source: Human HSP47, full length
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~47 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human
Antibody Positive Control: 1 µg/ml of SMC-203 was sufficient for detection of HSP47 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.
Antibody Species Reactivity: Human
Antibody Positive Control: 1 µg/ml of SMC-203 was sufficient for detection of HSP47 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.
Scientific Background: HSP47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of HSP47 around fibrotic lesions (1).
The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since HSP47 binds a single substrate (2). Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils (3).
References
[1] Razzaque MS., Taquchi T., (1999) Histol Histopathol. 14 (4): 1999-212.
[2] Taguchi T. et al. (2011) Acta Histochem Cytochem. 44(2):35-41.
[3] Nong Z. et al. (2011) Am J Pathol. 197(5): 2189-96.
[1] Razzaque MS., Taquchi T., (1999) Histol Histopathol. 14 (4): 1999-212.
[2] Taguchi T. et al. (2011) Acta Histochem Cytochem. 44(2):35-41.
[3] Nong Z. et al. (2011) Am J Pathol. 197(5): 2189-96.
© Kinexus Bioinformatics Corporation 2017