Product Name: HSP40
Product Number: AB-NN057-4
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Dna J protein homologue 1
Target Alias: DNAJ; DNAJ1; NDAJB1; HDJ1; HSP40; HSPF1; DnaJ homologue subfamily B member 1; Heat shock 40 kDa protein 1; HSP40; heat shock protein 40; Human DnaJ protein 1; hDj-1
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN057-4
Antibody Target Type: Pan-specific
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: C-terminal peptide of Pf11_0513 conjugated to KLH
Production Method: Protein A Affinity purified
Target Alias: DNAJ; DNAJ1; NDAJB1; HDJ1; HSP40; HSPF1; DnaJ homologue subfamily B member 1; Heat shock 40 kDa protein 1; HSP40; heat shock protein 40; Human DnaJ protein 1; hDj-1
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN057-4
Antibody Target Type: Pan-specific
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: C-terminal peptide of Pf11_0513 conjugated to KLH
Production Method: Protein A Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1.8 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Antibody Concentration: 1.8 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~ 62 kDa protein by Western blotting. Specific to P. Falciparum.
Antibody Species Reactivity: P. Falciparum
Antibody Positive Control: 0.9 µg/ml of SPC-184 was sufficient for detection of Pf11_0513 in 20 µg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: Does not cross-react to any protein from human erythrocytes.
Related Product 1: HSP40 pan-specific antibody (Cat. No.: AB-NN057-3)
Related Product 2: HSP40, Hdj1 pan-specific antibody (Cat. No.: AB-NN057-2)
Related Product 3: HSP40, YDJ1 pan-specific antibody (Cat. No.: AB-NN057-5)
Antibody Species Reactivity: P. Falciparum
Antibody Positive Control: 0.9 µg/ml of SPC-184 was sufficient for detection of Pf11_0513 in 20 µg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: Does not cross-react to any protein from human erythrocytes.
Related Product 1: HSP40 pan-specific antibody (Cat. No.: AB-NN057-3)
Related Product 2: HSP40, Hdj1 pan-specific antibody (Cat. No.: AB-NN057-2)
Related Product 3: HSP40, YDJ1 pan-specific antibody (Cat. No.: AB-NN057-5)
Scientific Background: DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s.
HSP40, also known as HDJ1 (1), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-6). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (3). PF11_0513 belongs to the HSP40 family of chaperones. This protein has a Plasmodium export element (PEXEL). It is exported out of the parasite into the infected erythrocytic compartment.
References
[1] Ohtsuka K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240.
[2] Melville, M. W. et al. (1997) PNAS USA, 94: 97-102.
[3] Hattori, H., Liu, Y-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K., and Ohtsuka, K. (1992) Cell Structure and Function 17: 77-86.
[4] Ohtsuka, K. Masuda, A., Nakai, A., and Nagata, K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647.
[5] Bardwell, J.C.A., Tilly, K., Craig, E., King, J., Zylicz, M. and Georgopoulos, C. (1986) J. Biol. Chem. 261: 1782-1785.
[6] Ohku, M., Tamura, F., Nishimura, S., and Uchida, H. (1986) J. Biol. Chem. 261: 1778-1781.
[1] Ohtsuka K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240.
[2] Melville, M. W. et al. (1997) PNAS USA, 94: 97-102.
[3] Hattori, H., Liu, Y-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K., and Ohtsuka, K. (1992) Cell Structure and Function 17: 77-86.
[4] Ohtsuka, K. Masuda, A., Nakai, A., and Nagata, K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647.
[5] Bardwell, J.C.A., Tilly, K., Craig, E., King, J., Zylicz, M. and Georgopoulos, C. (1986) J. Biol. Chem. 261: 1782-1785.
[6] Ohku, M., Tamura, F., Nishimura, S., and Uchida, H. (1986) J. Biol. Chem. 261: 1778-1781.
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