Product Name: HSP40
Product Number: AB-NN057-3
| Size: | 25 µl | Price: | 89.00 | |
| $US |
Target Full Name: DnaJ homologue subfamily B member 1
Target Alias: DnaJ (HSP40) homologue subfamily B member 1; DNAJ1; DNAJB1; HDJ1; HSPF1
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN057-3
Antibody Target Type: Pan-specific
Protein UniProt: P25685
Protein SigNET: P25685
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Recombinant purified human HSP40
Target Alias: DnaJ (HSP40) homologue subfamily B member 1; DNAJ1; DNAJB1; HDJ1; HSPF1
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN057-3
Antibody Target Type: Pan-specific
Protein UniProt: P25685
Protein SigNET: P25685
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Recombinant purified human HSP40
Production Method: Rabbit antiserum
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Storage Buffer: Rabbit antiserum
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Very high potency. Detects a ~40 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Storage Buffer: Rabbit antiserum
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Very high potency. Detects a ~40 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human
Antibody Positive Control: 0.5 µg/ml of SPC-100 was sufficient for detection of HSP40 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Related Product 1: HSP40 pan-specific antibody (Cat. No.: AB-NN057-4)
Related Product 2: HSP40, Hdj1 pan-specific antibody (Cat. No.: AB-NN057-2)
Related Product 3: HSP40, YDJ1 pan-specific antibody (Cat. No.: AB-NN057-5)
Related Product 4: HSP40, YDJ1 pan-specific antibody (Cat. No.: AB-NN057-6)
Antibody Specificity: Very high
Related Product 1: HSP40 pan-specific antibody (Cat. No.: AB-NN057-4)
Related Product 2: HSP40, Hdj1 pan-specific antibody (Cat. No.: AB-NN057-2)
Related Product 3: HSP40, YDJ1 pan-specific antibody (Cat. No.: AB-NN057-5)
Related Product 4: HSP40, YDJ1 pan-specific antibody (Cat. No.: AB-NN057-6)
Scientific Background: DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s.
HSP40, also known as HDJ1 (6), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-5). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (2).
Figure 1. Immunoblotting of various cell lines with AB-NN057-3 antibody at a 0.75 µg/mL final concentration. The target protein Hsp40 is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 65513.
Figure 2. Immunoblotting of various tissue lines with AB-NN057-3 antibody at a 0.75 µg/mL final concentration. The target protein Hsp40 is indicated. Each lane was loaded with 15 µg of tissue lysate protein. The max signal count was 65531.
References
[1] Melville, M. W. et al. (1997) PNAS USA, 94: 97-102.
[2] Hattori, H., Liu, Y-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K., and Ohtsuka, K. (1992) Cell Structure and Function 17: 77-86.
[3] Ohtsuka, K. Masuda, A., Nakai, A., and Nagata, K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647.
[4] Bardwell, J.C.A., Tilly, K., Craig, E., King, J., Zylicz, M. and Georgopoulos, C. (1986) J. Biol. Chem. 261: 1782-1785.
[5] Ohku, M., Tamura, F., Nishimura, S., and Uchida, H. (1986) J. Biol. Chem. 261: 1778-1781.
[6] Ohtsuka, K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240.
[1] Melville, M. W. et al. (1997) PNAS USA, 94: 97-102.
[2] Hattori, H., Liu, Y-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K., and Ohtsuka, K. (1992) Cell Structure and Function 17: 77-86.
[3] Ohtsuka, K. Masuda, A., Nakai, A., and Nagata, K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647.
[4] Bardwell, J.C.A., Tilly, K., Craig, E., King, J., Zylicz, M. and Georgopoulos, C. (1986) J. Biol. Chem. 261: 1782-1785.
[5] Ohku, M., Tamura, F., Nishimura, S., and Uchida, H. (1986) J. Biol. Chem. 261: 1778-1781.
[6] Ohtsuka, K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240.
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