Product Name: HO1 (Rat)
Product Number: AB-NN052-3
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Heme oxygenase 1
Target Alias: HSP32; HMOX1; HO; HO1
Product Type Specific: Heme oxygenase pan-specific antibody
Antibody Code: NN052-3
Antibody Target Type: Pan-specific
Protein UniProt: P09601
Protein SigNET: P09601
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1K
Antibody Immunogen Source: His-tagged Rat HO-1
Production Method: Protein G purified
Target Alias: HSP32; HMOX1; HO; HO1
Product Type Specific: Heme oxygenase pan-specific antibody
Antibody Code: NN052-3
Antibody Target Type: Pan-specific
Protein UniProt: P09601
Protein SigNET: P09601
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1K
Antibody Immunogen Source: His-tagged Rat HO-1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions should be determined by the user.
Antibody Potency: Detects a ~32 kDa protein in cell and tissue lysates by Western blotting.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions should be determined by the user.
Antibody Potency: Detects a ~32 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-234 was sufficient for detection of HO-1 in 10 µg of rat kidney lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: Does not cross-react with HO-2.
Related Product 1: HO1 pan-specific antibody (Cat. No.: AB-NN052-2)
Related Product 2: HO1 pan-specific antibody (Cat. No.: AB-NN052-4)
Related Product 3: HO1 (Rat) pan-specific antibody (Cat. No.: AB-NN052-5)
Related Product 4: HO1 pan-specific antibody (Cat. No.: AB-NN052-6)
Antibody Positive Control: 1 µg/ml of SMC-234 was sufficient for detection of HO-1 in 10 µg of rat kidney lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: Does not cross-react with HO-2.
Related Product 1: HO1 pan-specific antibody (Cat. No.: AB-NN052-2)
Related Product 2: HO1 pan-specific antibody (Cat. No.: AB-NN052-4)
Related Product 3: HO1 (Rat) pan-specific antibody (Cat. No.: AB-NN052-5)
Related Product 4: HO1 pan-specific antibody (Cat. No.: AB-NN052-6)
Scientific Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
References
[1] Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86.
[2] Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47.
[3] Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419.
[4] Brydun A. et al. (2007) Hypertens Res 30(4): 341-8.
[5] Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930.
[6] Yet S.F. et al. (2003) FASEB J. 17: 1759–1761.
[7] Yachie A. et al. (1999) J Clin Invest. 103: 129–135.
[1] Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86.
[2] Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47.
[3] Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419.
[4] Brydun A. et al. (2007) Hypertens Res 30(4): 341-8.
[5] Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930.
[6] Yet S.F. et al. (2003) FASEB J. 17: 1759–1761.
[7] Yachie A. et al. (1999) J Clin Invest. 103: 129–135.
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