Product Name: GRP94
Product Number: AB-NN049-4
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Endoplasmin
Target Alias: HSP90B1; GP96; TRA1; ECGP
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN049-4
Antibody Target Type: Pan-specific
Protein UniProt: P14625
Protein SigNET: P14625
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide corresponding to the sequence near the C-terminus of mouse GRP94
Production Method: Peptide Affinity purified
Target Alias: HSP90B1; GP96; TRA1; ECGP
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN049-4
Antibody Target Type: Pan-specific
Protein UniProt: P14625
Protein SigNET: P14625
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide corresponding to the sequence near the C-terminus of mouse GRP94
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IP (1:80); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium-high potency. Detects a ~94 kDa protein in cell and tissue lysates by Western blotting.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IP (1:80); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium-high potency. Detects a ~94 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Bovine
Antibody Positive Control: 1 µg/ml of SPC-101 was sufficient for detection of Grp94 in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-high
Related Product 1: GRP94 pan-specific antibody (Cat. No.: AB-NN049-3)
Antibody Positive Control: 1 µg/ml of SPC-101 was sufficient for detection of Grp94 in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-high
Related Product 1: GRP94 pan-specific antibody (Cat. No.: AB-NN049-3)
Scientific Background: Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5).
Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
References
[1] Rudolph R.W., and Bedows E. (1997) J Biol Chem 272: 3125-3128.
[2] Srivastava P.K. et al. (1994) Immunogenetics. 39(2):93-98.
[3] Mazzarella R.A., and Green M. (1987) J Biol Chem 262: 8875-8883.
[4] Kang, H.S. and Welch W.J. (1991) J Biol Chem 266(9): 5643-5649.
[5] Chu F. et al. (2006) Protein Sci 15(6): 1260-1269.
[6] Peter F. et al., (1992) J Biol Chem 267: 10631-10637.
[7] Allen S. et al. (2000) Blood 96(2): 560-568.
[8] Sato K et al. (2001) Blood 98(6): 1852-1857.
[1] Rudolph R.W., and Bedows E. (1997) J Biol Chem 272: 3125-3128.
[2] Srivastava P.K. et al. (1994) Immunogenetics. 39(2):93-98.
[3] Mazzarella R.A., and Green M. (1987) J Biol Chem 262: 8875-8883.
[4] Kang, H.S. and Welch W.J. (1991) J Biol Chem 266(9): 5643-5649.
[5] Chu F. et al. (2006) Protein Sci 15(6): 1260-1269.
[6] Peter F. et al., (1992) J Biol Chem 267: 10631-10637.
[7] Allen S. et al. (2000) Blood 96(2): 560-568.
[8] Sato K et al. (2001) Blood 98(6): 1852-1857.
[9] Yun S.-W. et al (2000) Brain Research Bulletin.52(5): 371-378.
[10] Choukhi A. et al. (1998) J. Virol. 72: 3851-3858.
[11] Hoshino T. et al. (1998) Blood 91(11): 4379-4386; Riera M. et al. (1999) Mol. Cell Biochem. 191: 97-104; Gusarova V. et al. (2001) J. Biol. Chem. 276(27): 24891-24900.
[10] Choukhi A. et al. (1998) J. Virol. 72: 3851-3858.
[11] Hoshino T. et al. (1998) Blood 91(11): 4379-4386; Riera M. et al. (1999) Mol. Cell Biochem. 191: 97-104; Gusarova V. et al. (2001) J. Biol. Chem. 276(27): 24891-24900.
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