Product Name: GRP78 (Bip)
Product Number: AB-NN048-2
| Size: | 50 µl | Price: | 89.00 | |
| $US |
Target Full Name: 78 kDa glucose-regulated protein
Target Alias: BIP; Grp78; HspA5; MIF2; immunoglobulin heavy chain binding protein
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN048-2
Antibody Target Type: Pan-specific
Protein UniProt: P11021
Protein SigNET: P11021
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Rat GRP78 (Bip) synthetic peptide conjugated to KLH
Production Method: Rabbit antiserum
Target Alias: BIP; Grp78; HspA5; MIF2; immunoglobulin heavy chain binding protein
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN048-2
Antibody Target Type: Pan-specific
Protein UniProt: P11021
Protein SigNET: P11021
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Rat GRP78 (Bip) synthetic peptide conjugated to KLH
Production Method: Rabbit antiserum
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Storage Buffer: Rabbit antiserum
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting, Immunofluorescence-p (1:100)
Antibody Dilution Recommended: 1:2000 (WB)
Antibody Potency: Very high potency. Detects a protein with a mass of ~78 kDa corresponding to Grp78 in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human, Mouse, Rat, Cow, Fungus (Cryphonectria parasitica), Hamster, Monkey, Rabbit, Xenopus
Storage Buffer: Rabbit antiserum
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting, Immunofluorescence-p (1:100)
Antibody Dilution Recommended: 1:2000 (WB)
Antibody Potency: Very high potency. Detects a protein with a mass of ~78 kDa corresponding to Grp78 in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human, Mouse, Rat, Cow, Fungus (Cryphonectria parasitica), Hamster, Monkey, Rabbit, Xenopus
Antibody Positive Control: 0.5 μg/ml of SPC-107 was sufficient for detection of Grp78 in 10ug of rat tissue lysate by ECL immunoblot analysis.
Related Product 1: GRP78 pan-specific antibody (Cat. No.: AB-NN048-3)
Related Product 2: GRP78 pan-specific antibody (Cat. No.: AB-NN048-4)
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Related Product 4: GRP78 pan-specific antibody (Cat. No.: AB-NN048-6)
Related Product 5: GRP78 pan-specific antibody (Cat. No.: AB-NN048-8)
Related Product 6: GRP78 (Bip) pan-specific antibody (Cat. No.: AB-NN048-7)
Related Product 1: GRP78 pan-specific antibody (Cat. No.: AB-NN048-3)
Related Product 2: GRP78 pan-specific antibody (Cat. No.: AB-NN048-4)
Related Product 3: GRP78 pan-specific antibody (Cat. No.: AB-NN048-5)
Related Product 4: GRP78 pan-specific antibody (Cat. No.: AB-NN048-6)
Related Product 5: GRP78 pan-specific antibody (Cat. No.: AB-NN048-8)
Related Product 6: GRP78 (Bip) pan-specific antibody (Cat. No.: AB-NN048-7)
Scientific Background: GRP78 is a ubiquitously expressed, 78-kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
Figure 1. Immunoblotting of various cell lines with AB-NN048-2 antibody at a 1000 X dilution. The target protein Grp78 is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 60919.
Figure 2. Immunoblotting of various tissue lines with AB-NN048-2 antibody at a 1000 X dilution. The target protein Grp78 is indicated. Each lane was loaded with 15 µg of tissue lysate protein. The max signal count was 57993.
References
[1] Cho S. et al. (2007). Mol Cell Biol 27(1): 368-83.
[2] Yang Y. et al. (1998) J Biol Chem 273: 25552-25555.
[3] Luo S., et al (2006) 26 (15): 5688-97.
[4] Yu Z. et al. (1999) Exp Neurol. 15: 302-314.
[5] Koomagi R. et al. (1999) Anticancer Res. 19:4333- 4336.
[6] Laquerre S. et al. (1998) J. Virology 72: 4940-4949.
[7] Dong D. et al. (2005) Cancer Res 65(13): 5785-91.
[1] Cho S. et al. (2007). Mol Cell Biol 27(1): 368-83.
[2] Yang Y. et al. (1998) J Biol Chem 273: 25552-25555.
[3] Luo S., et al (2006) 26 (15): 5688-97.
[4] Yu Z. et al. (1999) Exp Neurol. 15: 302-314.
[5] Koomagi R. et al. (1999) Anticancer Res. 19:4333- 4336.
[6] Laquerre S. et al. (1998) J. Virology 72: 4940-4949.
[7] Dong D. et al. (2005) Cancer Res 65(13): 5785-91.
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