Product Name: Grp75
Product Number: AB-NN047-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Stress-70 protein, mitochondrial
Target Alias: HSC74; HSP74; HSPA9; HSPa9a; HSPA9B; Mortalin 2; MOT2; PBP74
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN047-2
Antibody Target Type: Pan-specific
Protein UniProt: P38646
Protein SigNET: P38646
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 551-766 of mouse SALM2.
Production Method: Protein G purified
Target Alias: HSC74; HSP74; HSPA9; HSPa9a; HSPA9B; Mortalin 2; MOT2; PBP74
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN047-2
Antibody Target Type: Pan-specific
Protein UniProt: P38646
Protein SigNET: P38646
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 551-766 of mouse SALM2.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Antibody Potency: Very high potency. Detects a ~75 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | C.elegans
Antibody Positive Control: 1 µg/ml was sufficient for detection of Grp75 in 10 µg of heat shock HeLa lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Antibody Specificity: Very high
Antibody Species Reactivity: Human | Mouse | Rat | C.elegans
Antibody Positive Control: 1 µg/ml was sufficient for detection of Grp75 in 10 µg of heat shock HeLa lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Antibody Specificity: Very high
Scientific Background: Grp75, also known as mortalin, is a member of HSP70 family of chaperone proteins that is not heat inducible (1, 2). Grp75 is actually induced under conditions of low glucose and other nutritional and environmental stresses. Grp75 resides primarily in the mitochondrial matrix, where it collaborates with HSP60 in the re-folding of proteins translocated into this organelle (3, 4). Related forms may also be found in the cytosol or on the surface of the extracellular membrane.
Other Grp75 functions include its ability to inactivate the tumor suppressor p53 (5). Studies have found that Grp75 is over-expressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (6). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (7). And finally, like its E.coli homolog DnaK (8), GRP75 possesses a cation-dependent ATPase activity considered central to its function as a chaperone (9, 10).
References
[1] Kaul S.C. et al. (1993) Biochem Biophys Res Commun. 193: 348-355.
[2] Wadhwa R. et al. (1993) J Biol Chem 268: 6615-6621.
[3] Schneider H.C. et al. (1994) Nature 371: 768-774.
[4] Manning-Krieg U.C. et al. (1991) EMBO J. 10: 3273-3280.
[5] Wadhwa R. et al. (1998) J Biol Chem. 273: 29586-91.
[6] Wadhwa R. et al. (2006) Int J Cancer 118: 2973-2980.
[7] Kaul S.C. et al. (2003) Exp Cell Res. 286: 96-110.
[8] Liberek K. et al. (1991) J Biol Chem. 266: 14491-14496.
[1] Kaul S.C. et al. (1993) Biochem Biophys Res Commun. 193: 348-355.
[2] Wadhwa R. et al. (1993) J Biol Chem 268: 6615-6621.
[3] Schneider H.C. et al. (1994) Nature 371: 768-774.
[4] Manning-Krieg U.C. et al. (1991) EMBO J. 10: 3273-3280.
[5] Wadhwa R. et al. (1998) J Biol Chem. 273: 29586-91.
[6] Wadhwa R. et al. (2006) Int J Cancer 118: 2973-2980.
[7] Kaul S.C. et al. (2003) Exp Cell Res. 286: 96-110.
[8] Liberek K. et al. (1991) J Biol Chem. 266: 14491-14496.
[9] Mizzen L.A. et al. (1991) Cell Regulation. 2: 165-179.
[10] Leustek U.K. et al. (1989) PNAS USA. 86: 7805-7808.
[10] Leustek U.K. et al. (1989) PNAS USA. 86: 7805-7808.
© Kinexus Bioinformatics Corporation 2017