Product Name: LRRK2/Dardarin
Product Number: AB-NK303-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Leucine-rich repeat serine/threonine-protein kinase 2
Target Alias: Leucine-rich repeat kinase 2; RIP7; PARK8; Dardarin; ROCO 2; RIPK7; augmented in rheumatoid arthritis 17
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK303-1
Antibody Target Type: Pan-specific
Protein UniProt: Q5S007
Protein SigNET: Q5S007
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Target Alias: Leucine-rich repeat kinase 2; RIP7; PARK8; Dardarin; ROCO 2; RIPK7; augmented in rheumatoid arthritis 17
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK303-1
Antibody Target Type: Pan-specific
Protein UniProt: Q5S007
Protein SigNET: Q5S007
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Fusion protein amino acids 841-960 of human LRRK2. 81% identical in mouse, 80% identical in rat. <30% identity with LRRK1.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a >200 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Rat
Antibody Positive Control: 10 µg/ml of SMC-445 was sufficient for detection of LRRK2/Dardarin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: LRRK2/Dardarin pan-specific antibody (Cat. No.: AB-NK303-2)
Related Product 2: LRRKtide KinSub - LRRKtide peptide substrate
Related Product 3: LRRKSubtide - LRRK1 protein kinase substrate peptide
Antibody Potency: Detects a >200 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Rat
Antibody Positive Control: 10 µg/ml of SMC-445 was sufficient for detection of LRRK2/Dardarin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: LRRK2/Dardarin pan-specific antibody (Cat. No.: AB-NK303-2)
Related Product 2: LRRKtide KinSub - LRRKtide peptide substrate
Related Product 3: LRRKSubtide - LRRK1 protein kinase substrate peptide
Scientific Background: LRRK2 is a large protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named Roc, and a kinase domain that is closely related to the RIP kinase domain. LRRK2 gene is expressed in brain as well as in other tissues such as lung, liver and heart. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with parkinsonism. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features.
© Kinexus Bioinformatics Corporation 2017