Product Name: CaMKII (Alpha-Specific)
Product Number: AB-NK302-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
Target Alias: Calcium/calmodulin-dependent protein kinase II alpha-B subunit; Calcium/calmodulin-dependent protein kinase type II alpha chain; CaM kinase II alpha subunit; CAMK2A; CAMKA; CaMK-II alpha subunit; CaMKII-alpha; CaMKIINalpha; CaM-kinase II alpha chain; EC 2.7.11.17; KCC2A; KIAA0968
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK302-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UQM7
Protein SigNET: Q9UQM7
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Target Alias: Calcium/calmodulin-dependent protein kinase II alpha-B subunit; Calcium/calmodulin-dependent protein kinase type II alpha chain; CaM kinase II alpha subunit; CAMK2A; CAMKA; CaMK-II alpha subunit; CaMKII-alpha; CaMKIINalpha; CaM-kinase II alpha chain; EC 2.7.11.17; KCC2A; KIAA0968
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK302-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UQM7
Protein SigNET: Q9UQM7
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Partially purified rat CaMKII
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | RIA/ELISA
Antibody Immunogen Source: Partially purified rat CaMKII
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | RIA/ELISA
Antibody Dilution Recommended: WB (1:10000), IHC (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~50-60 kDa protein in cell and tissue lysates by Western blotting. Recognizes both phosphorylated and non-phosphorylated forms.
Antibody Species Reactivity: Human | Mouse | Rat | Bovine
Antibody Positive Control: 0.1 µg/ml was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Antibody Potency: Detects a ~50-60 kDa protein in cell and tissue lysates by Western blotting. Recognizes both phosphorylated and non-phosphorylated forms.
Antibody Species Reactivity: Human | Mouse | Rat | Bovine
Antibody Positive Control: 0.1 µg/ml was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Scientific Background: CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
References
[1] Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–3601
[2] Barria A. et al. (1997) Science 276: 2042–2045.
[4] Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808.
[5] Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479.
[6] Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102.
[7] Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292.
[8] Means A.R. (2000) Mol. Endocrinol. 14: 4–12.
[9] Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510.
[1] Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–3601
[2] Barria A. et al. (1997) Science 276: 2042–2045.
[4] Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808.
[5] Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479.
[6] Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102.
[7] Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292.
[8] Means A.R. (2000) Mol. Endocrinol. 14: 4–12.
[9] Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510.
[10] Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692.
[11] Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244.
[11] Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244.
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